一株乙醇降解菌株的分离和鉴定  

Isolation and identification of a bacterial strain with potentiality in ethanol and aldehyde detoxification

作　　者：赵玉凤[1,2] 雷明科[2] 吴元欣[2] 王存文[2] 

机构地区：[1]华中农业大学生命科学技术学院农业微生物学国家重点实验室,武汉430070 [2]武汉工程大学化工与制药学院,绿色化工过程省部共建教育部重点实验室,湖北省新型反应器与绿色化学工艺重点实验室,武汉430074

出　　处：《工业微生物》2009年第1期56-59,共4页Industrial Microbiology

基　　金：湖北省国际科技合作重点项目计划(项目编号2006CA013);湖北省科技攻关计划(项目编号:2007AA201C27;2007AA301824)

摘　　要：从湖北农田土壤中筛选得到一株ALDH活性较高的菌株,该菌株在含0.64%乙醇的培养基中生长较佳,且耐受0.9%的乙醛。经菌种形态学和生理生化特征,以及16S rRNA基因序列分析,鉴定该菌株为不动杆菌(Acinetobacter sp.)。该菌株在乙醇和乙醛解毒研究中有重要价值。A bacterial strain with high acetaldehyde dehydrogenase （ALDH） activity were screened from soil in Hubei province. The strain grew well on meat-peptone medium with 0.64% of ethanol. And it could live on plate with acetaldehyde content up to 0.9 %. The bacterium was identified as Acinetobacter sp. based on its phenotypical and biochemical properties as well as its partial 16S rRNA gene sequence. This strain was useful in ethanol and acetaldehyde detoxification.

关 键 词：乙醛脱氢酶 16S RRNA基因 不动杆菌 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ223.122