茶树β-葡萄糖苷酶和β-樱草糖苷酶基因表达差异分析  

Differential Expression Analysis of β-glucosidase and β-primeverosidase Genes in Tea Plant (Camellia sinensis)

作　　者：陈亮[1] 赵丽萍[1] 马春雷[1] 王新超[1] 姚明哲[1] 

机构地区：[1]中国农业科学院茶叶研究所茶树资源与改良研究中心/国家茶树改良中心,杭州310008

出　　处：《园艺学报》2009年第1期87-92,共6页Acta Horticulturae Sinica

基　　金：国家‘863’计划项目(2006AA10Z171);浙江省钱江人才计划项目(2006R10042);‘十一五’国家科技支撑计划项目(2006BAD13B06-1)

摘　　要：β-葡萄糖苷酶和β-樱草糖苷酶是茶叶香气释放过程中2个重要的内源性水解酶。采用实时定量PCR技术,分析了‘龙井43’茶品种春季不同嫩度新梢,以及8个不同品种春季一芽二叶的β-葡萄糖苷酶和β-樱草糖苷酶基因的表达差异。结果表明,β-葡萄糖苷酶基因表达量以一芽三叶最高,为5.83×107copy.μL-1,一芽三叶>一芽一叶>一芽二叶>一芽四叶;β-樱草糖苷酶基因在一芽一叶表达量很低,一芽二叶最高(2.49×106copy.μL-1),而后随着成熟度的增加而降低。β-葡萄糖苷酶基因的表达量明显高于β-樱草糖苷酶基因,一芽二叶和一芽三叶新梢中两个基因的表达量均较高。8个不同品种中,‘福鼎大白茶’2个基因表达量均非常高。8个品种β-葡萄糖苷酶基因表达量是β-樱草糖苷酶的20.6(2.4～45.6)倍。不过,‘龙井43’不同时空、不同茶树品种β-葡萄糖苷酶活性测定结果与基因表达量之间的相关性均不明显。β-glucosidase and β-primeverosidase are two important endogenous hydrolytic enzymes for the formation of tea aroma. Real-time PCR technique was employed to analyze the differential expression of two aroma related genes, β-glucosidase and β-primeverosidase genes, in different maturity shoots and different source cultivars in spring season of tea plant （ Camellia sinensis）. The results indicated that the highest expression level for β-glucosidase gene was found in ‘three and a bud＇ （5.83×10^7copy·μL^-1）. The expression level order of β-glucosidase gene was ‘three and a bud＇ 〉 ‘one and a bud＇ 〉 ‘two and a bud＇ 〉 ‘four and a bud＇. For β-primeverosidase gene, expression level in ‘one and a bud＇ was very low, the high- est was found in ‘two and a bud＇ （2.49×10^7copy·μL^-1）. The expression level decreased with young shoot becoming mature. The expression level of β-glucosidase gene is higher compared with that of β-primeverosidase gene in tea plant. Both genes are highly expressed in ‘two and a bud＇ or ‘three and a bud＇ shoots. The genes expression level of 8 tea cultivars, which are typical clones suitable for green, Oolong and black teas from China, Japan and Kenya, were assessed. Both genes were very highly expressed in Fuding Dabaicha. The ratio of expression level for the two genes in 8 different cultivars was 20.6（2.4 -45.6） folders. Never-theless, the gene expression level and enzyme activity of 13-glucosidase in different soace-time of Longjing 43,as well as different source cultivars were inconsistent.

关 键 词：茶树 香气 基因 表达差异 

分 类 号：S571.1[农业科学—茶叶生产加工]