利用七叶灵显色技术检验和判断β-葡萄糖苷酶的研究  

Detection and Identification of β-glucosidase with Esculin as Substrste

作　　者：赵林果[1] 孟鹏[1] 李丽娟[1] 余世袁[1] 

机构地区：[1]南京林业大学化学工程学院生物工程系

出　　处：《食品与发酵工业》2008年第12期163-166,共4页Food and Fermentation Industries

基　　金：国家教育部科学技术研究重点项目(No.204054);江苏省自然科学基金(No.BK2008423)

摘　　要：七叶灵(6,7-2-羟基-香豆素-β-D-葡萄糖苷)在β-葡萄糖苷酶的作用下能生成葡萄糖和七叶苷原(6,7-2-羟基-香豆素),七叶苷原能和Fe3+作用呈现棕黑色。七叶灵显色技术用于β-葡萄糖苷酶高产菌株的筛选、β-葡萄糖苷酶提纯时的样品检测、非变性聚丙烯酰胺凝胶活性染色的应用结果表明,以七叶灵为作用底物检验和判断β-葡萄糖苷酶的新方法具有高分辨率和灵敏性,而且操作简易、快速、经济。Based on the well-established reaction of β-glucosidase with eseulin （6,7- dihydroxycoumarin - β-glucoside）, Esculin were used as a substrate for Screening of the strain producing β-glucosidase from a large number of organisms, detection β-glucosidase from Chromatographic fractions and identification β-glucosidase in polyacrylamide gels. The experiment results showed that it was a novel technique for the detection and identification of β-glucosidase with high resolution and sensitivity and that can be performed easily, rapidly, and at a minimal cost.

关 键 词：Β-葡萄糖苷酶 检验 七叶灵 新方法 

分 类 号：TS201.2[轻工技术与工程—食品科学]