Cell adhesion molecules regulate contractile ring-indepen- dent cytokinesis in Dictyostelium discoideum  

作　　者：Akira Nagasaki Masamitsu Kanada Taro QP Uyeda 

机构地区：[1]Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8562, Japan [2]Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305- 8572, Japan [3]Biomedicinal Information Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Koto, Tokyo 135-0064, Japan

出　　处：《Cell Research》2009年第2期236-246,共11页细胞研究（英文版）

摘　　要：To investigate the roles of substrate adhesion in cytokinesis, we established cell lines lacking paxillin （PAXB） or vineulin （VINA）, and those expressing the respective GFP fusion proteins in Dictyostelium discoideum. As in mammalian cells, GFP-PAXB and GFP-VINA formed focal adhesion-like complexes on the cell bottom, paxB^- cells in suspension grew normally, but on substrates, often failed to divide after regression of the furrow. The efficient cytokinesis of paxB^- cells in suspension is not because of shear forces to assist abscission, as they divided normally in static suspension culture as well. Double knockout strains lacking mhcA, which codes for myosin II, and paxB or vinA displayed more severe cytokinetic defects than each single knockout strain. In mitotic wild-type cells, GFP-PAXB was diffusely distributed on the basal membrane, hut was strikingly condensed along the polar edges in mitotic mhcA^- cells. These results are consistent with our idea that Dictyostelium displays two forms of cytokinesis, one that is contractile ringdependent and adhesion-independent, and the other that is contractile ring-independent and adhesion-dependent, and that the latter requires PAXB and VINA. Furthermore, thatpaxB- cells fail to divide normally in the presence of substrate adhesion suggests that this adhesion molecule may play additional signaling roles.

关 键 词：DICTYOSTELIUM PAXILLIN VINCULIN CYTOKINESIS cell shapes and cell motility 

分 类 号：Q24[生物学—细胞生物学]