骨髓增生异常综合征患者骨髓高分辨染色体的制备与分析  

Application of high-resolution chromosome preparation and research in 160 patients with MDS

作　　者：张云普[1] 邹典斌[2] 李锦堂[2] 

机构地区：[1]郑州市第三人民医院,郑州450000 [2]郑州大学第一附属医院血液科,郑州450052

出　　处：《郑州大学学报（医学版）》2009年第1期186-187,共2页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:了解高分辨染色体的制备方法在骨髓增生异常综合征(MDS)患者染色体核型分析中的应用价值。方法:随机选择160例MDS患者,同时应用常规方法和高分辨染色体的制备方法制备骨髓染色体。常规染色体的制备:患者骨髓经短期(24h)培养后,加入秋水仙胺,37℃孵育1h后收获细胞。骨髓高分辨染色体的制备:取初诊MDS患者骨髓,按有核细胞(1.5～2.0)×106mL-1接种于RPMI1640培养基中,置37℃恒温箱中,培养48h。在收获前2h加溴化乙锭(EB),再于收获前1h加入秋水仙胺,按常规方法制片,G-显带。以同期住院的40例非恶性血液系统疾病患者为对照。结果:应用高分辨染色体制备方法制备出的骨髓染色体,每一单倍体带达550条左右。在160例MDS患者中,共发现90例骨髓染色体数目或结构异常,其中-5/5q-17例,-7/7q-31例,-Y11例,t(9,22)15例,其余16例为4、6、10、11号染色体异常。应用常规染色体制备方法制备的骨髓染色体核型分析结果与高分辨染色体方法制备的骨髓染色体相同,但每一单倍体带在400条左右。40例非恶性血液病患者骨髓染色体未发现数目和结构异常。结论:骨髓高分辨染色体可检出更多的异常染色体,并可确定精细的断裂点。Aim： To analyse the karyotype of patients with myelodysplastic syndromes （MDS）by high resolution banding chromosome technique. Methods： Routine and high resolution banding chromosome technique were applied to analyse to 160 patients with MDS and 40 cases normal controls. Conventional chromosome preparation, the bone marrow cells of patients after short-term（24 h）cluture, Adding colchicine amine, were received after 1 h incubation, 37 ℃. High-resolution bone marrow chromosome preparation, bone marrow Fells were raked in newly diagnosed patients with MDS, according to the nucleated ceils （1.5 -2.0） × 10^6 mL^-1 coverage in the medium RPMI 1640, 37 ℃, incubating 48h, adding ethidum bromide （EB） precede receive, adding colcbicinc precede receive, according to the conventional method of production, G- banding. 40 patients with non-malignant blood disease stayed in hospital in the same period are controls. Results： Improved high-resolution technique EB induced method combined with the method of colchicine inhibition was used to chem high resolution banding chromosome （about 550 bands）. 90 cases patients with MDS had chromosome numerical or structural aberration. 17 cases with -5/5q - , 31 cases with -7/7 q -. 11 cases with -Y, 15 cases with t（9; 22） , The other 16 cases were 4, 6, 10, 11 chromosome abnormalities. The same results were obtained by routine methed with 400 hands. Chromosome oberration were not found in 40 normal controls. Conclusion ： High-resolution banding method can detect more chromosome abnormalities and to determine subtle breaking speck.

关 键 词：骨髓增生异常综合征 高分辨染色体 核型分析 

分 类 号：R733.7[医药卫生—肿瘤]