紫外线诱变青霉P-M1选育柚苷酶高产菌株研究  

Breeding of High-yield Naringinase Strain from Penicillium sp. P-M1 by UV Mutation

作　　者：崔培梧[1] 黎继烈[1] 文蓉[1] 黄凌[1] 杨杰[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院发酵工程实验室,湖南长沙412006

出　　处：《北方园艺》2009年第2期42-44,共3页Northern Horticulture

摘　　要：采用紫外线对野生型柚苷酶产生菌青霉P-M1进行诱变处理以提高柚苷酶的产量,经过初步筛选最佳诱变条件为:孢子悬液(107个/mL)稀释度为10-5,15 W紫外灯,垂直照射距离为30 cm,处理时间为5 min。通过透明圈初筛和摇瓶复筛选育出一株柚苷酶高产菌株青霉PU-15,酶活达到334.3 U/mL,比出发菌株提高了137.4%,将其连续传代10次测定遗传稳定性,结果显示稳定性良好,是一株比较理想的柚苷酶高产菌株。For increasing the yield of naringinase which has great potential in the debittering of citrus the wild Penicillium sp. P-M1 was treated with ultraviolet（UV） radiation and the optimum treating conditions which was got by primary screening were as follows： the dilution of the spore suspension（10^7/mL） was 10^-5, the 15W UV lamp was used as UV light with 30 cm radiation distance and 5 minutes treating time. Through UV mutation a mutant strain named PU-15 was obtained. As compared with the initial strain, the activity of naringinase had reached 334.3 U/mL which was 137.4% higher than the initial strain. Then an experiment for studying the genetic stability was took by means of passaging 5 times and its results showed that Penicillium sp. PU-15 had a better genetic stability.

关 键 词：柚苷酶 青霉 紫外诱变 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]