超高效液相色谱-串联质谱法检测动物源食品中8种氨基糖苷类药物残留  

Determination of Aminoglycosides in Foodstuffs of Animal Origin by UPLC-MS/MS

作　　者：孙雷[1] 张骊[1] 黄耀凌[1] 汪霞[1] 王树槐[1] 

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《质谱学报》2009年第1期60-64,共5页Journal of Chinese Mass Spectrometry Society

基　　金：国家科技支撑计划课题项目(No:2006BAD31B09)资助

摘　　要：建立了动物源食品中大观霉素，链霉素，双氢链霉素，阿米卡星，卡那霉素，安普霉素，庆大霉素和新霉素等8种氨基糖苷类药物残留检测的超高效液相色谱-串联质谱（UPLC-MS／MS）方法。结果表明：8种氨基糖苷类药物在0．05～2mg·L^-1浓度范围内呈现良好的线性关系，相关系数R^2均大于0．998；方法检测限为5μg·kg^-1，定量限为10μg·kg^-1；从100、200和300μg·kg^-1 3个添加浓度检测结果可以看出，方法平均回收率为83．0％～114．1％，批内RSD为1．1％～11．4％，批间RSD为2．1％～8．9％，可以满足食品安全有关法规的要求。Spectinomycin, streptomycin, dihydrostreptomycin, amikacin, kanamycin, apramycin, gentamicin and neomycin in foodstuffs of animal origin were detected by UPLC-MS/ MS. The calibration curve is good linear between the peak areas and the concentrations of 0.05-2 mg · L^-1 ,the correlation coefficient R^2〉0. 998. The limit of detection is 5 μg· kg^-1 , and the limit of quantification is 10 μg·kg^-1. The average recoveries from spiked milk at the three concentrations of 100, 200 and 300 μg ·kg^-1 range from 83.0-114. 1%, and intra- and inter-batch variation coefficients are 1.1%-11.4% and 2.1-8. 9%, respectively.

关 键 词：超高效液相色谱-串联质谱法 动物源食品 氨基糖苷类药物 

分 类 号：O657.63[理学—分析化学]