壳聚糖对茶树抗性酶调节作用的研究  

Effect of chitosan on activities of enzymes relating to resistance of tea plant

作　　者：孙世利[1,2] 骆耀平[1] 

机构地区：[1]浙江大学农业与生物技术学院茶学系,浙江杭州310029 [2]广东省农业科学院茶叶研究所,广东广州510640

出　　处：《浙江大学学报（农业与生命科学版）》2009年第1期84-88,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省教育厅资助项目(20030255);浙江省武义县科技局资助项目(2004-5-24)

摘　　要：为研究壳聚糖对茶树抗逆性的作用,选取浙农21、浙农113和浙农1393个茶树品种,经不同浓度的壳聚糖喷施茶树叶片处理后,分别测定茶树叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)的活性.结果表明,100、200、300mg·L-1的壳聚糖均可使3个茶树品种叶片中的抗性相关酶活性发生变化.浙农21、浙农113和浙农1393个茶树品种分别在200、100、200mg·L-1壳聚糖处理时SOD活性最高,与对照相比分别提高106.87%、58.55%、68.2%;在200、200、300mg·L-1壳聚糖处理时POD活性最高,比对照分别提高18.47%、20%、18.68%;在200、300、100mg·L-1壳聚糖处理时PAL活性最高,比对照分别提高33.55%、12.16%和31.33%.表明壳聚糖对提高茶树的抗逆能力具有潜在的调节作用.Effects of chitosan on activities of super oxide dismutase （SOD）, peroxidase （POD） and phenylalanine ammonia lyase （PAL） by foliar spraying in tea plants （Camellia sinen.sis cv. Zhenong 21, Zhenong 113 and Zhenong 139） were investigated. The results show that activities of SOD, POD and PAL in tea leaves were influenced by chitosan. The responses of the enzymes in various tea cultivars to chitosan were differentiated. SOD activities of Zhenong 21, Zhenong 113 and Zhenong 139 were increased by 106.87%, 58.55% and 68.2% when chitosan concentrations were at 200, 100, 200 mg·L^-1, respectively. POD activities were increased by 18. 47%, 20% and 18. 68% when chitosan concentrations were at 200, 200, 300mg·L^-1, respectively. PAL activities were increased by 33.55%, 12.16% and 31.33% when chitosan concentrations were at 200, 300, 100 mg·L^-1, respectively. The study suggests that chitosan might have the potential to enhance the stress resistance of tea plants.

关 键 词：壳聚糖 茶树 抗性 酶 

分 类 号：S571.1[农业科学—茶叶生产加工]