大蒜新素对脑缺血再灌注大鼠海马的保护作用及对P53蛋白表达的影响  

Protective effect of allitridi on hippocampus of rats with cerebral ischemia-reperfusion and P53 expression

作　　者：郭莉华[1] 李清君[2] 胡玉燕[2] 褚赛纯[2] 李文斌[2] 张景坤[2] 

机构地区：[1]邢台医学高等专科学校,河北邢台054000 [2]河北医科大学病理生理学教研室,河北石家庄050017

出　　处：《中国病理生理杂志》2009年第2期264-267,共4页Chinese Journal of Pathophysiology

摘　　要：目的:观察大蒜新素对脑缺血再灌注大鼠海马神经元的保护作用及对海马P53蛋白表达的影响。方法:采用大鼠全脑缺血模型(4VO法),大蒜新素10、20和30mg/kg分两次于缺血前30min和再灌注10min时经尾静脉注入,每次注射总量的1/2。再灌注24h时取大鼠海马,甲苯胺蓝染色观察海马CA1区神经元的形态学改变,流式细胞技术检测海马神经元凋亡率,免疫组织化学方法检测海马CA1区P53蛋白的表达。结果:与sham组比较,大鼠全脑缺血10min再灌注24h时,海马CA1区部分神经元死亡,存活神经元数目明显减少,海马神经元凋亡率明显增高,P53蛋白表达增多。静脉给予大蒜新素可使缺血再灌注大鼠海马组织损伤程度减轻,存活神经元数目增加,神经元凋亡率降低,P53蛋白表达减少。结论:大蒜新素对脑缺血再灌注大鼠海马神经元具有保护作用,通过下调P53蛋白的表达而抑制神经元凋亡可能是其发挥保护作用的重要机制之一。AIM ： To observe the protective effect of allitridi on hippocampal neuron of rats with cerebral ischemia- reperfusion （I/R） injury and to investigate its effects on P53 expression in hippocampus. METHODS： The global cerebral ischemia - reperfusion models were established by 4 - vessel occlusion. Allitridi at doses of 10, 20 or 30 mg/kg was injected through rat＇ s tail vein, half dose at 30 min before brain ischemia and another half dose at 10 min after reperfusion were injected, respectively. The hippocampus of rat was removed 24 h after reperfusion. Toluidine blue staining was applied to estimate morphologic changes. Flow cytometry was used to evaluate neuronal apoptosis rate of hippocampus. Immunohistochemistry was used to observe the expression of P53 protein. RESULTS： Compared with sham group, survival neuronal density in I/＇R group was significantly depressed. The rate of neuronal apoptosis and the expression of P53 protein were significantly increased. Allitridi significantly increased the number of survival neurons in hippocampus compared to l/ R group. Meanwhile, allitridi remarkably inhibited the rate of neuronal apoptosis and the expression of P53 protein. CONCLUSION ： Allitridi has protective role against brain ischemia reperfusion injury. The mechanism may be involved in blocking P53 protein expression in hippocampus of rats with ischemia - reperfusion.

关 键 词：大蒜新素 脑缺血 海马 蛋白质P53 神经元凋亡 

分 类 号：R363.2[医药卫生—病理学]