黄芩苷元对炎症反应的影响  

Effects of baicalein on inflammation

作　　者：朱伟[1] 孙红光[2] 朱迅[1] 

机构地区：[1]海南大学海洋学院制药工程系,海南省热带水生生物技术重点实验室,海南海口570228 [2]吉林大学基础医学院免疫教研室,吉林长春130021

出　　处：《中国药理学通报》2009年第2期194-197,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30371334);海南省自然科学基金资助项目(No80413);海南大学自然科学基金资助项目(Nokyjj0430)

摘　　要：目的研究以黄芩苷元为主要成分的黄芩提取物SBM抗炎作用,并初步探讨其作用机制。方法观察SBM体外对PGE2合成、COX-2酶活性,COX-2蛋白表达、p38蛋白磷酸化的影响;观察给药后对角叉菜胶诱导大鼠足肿胀、醋酸诱导的小鼠扭体反应的影响。结果SBM可抑制LPS诱导腹腔巨噬细胞PGE2合成、COX-2酶活性及COX-2蛋白表达;抑制ConA诱导的小鼠脾淋巴细胞p38蛋白磷酸化;并可抑制角叉菜胶诱导的大鼠足肿胀及醋酸诱导的小鼠扭体反应。结论黄芩提取物SBM可通过抑制淋巴细胞功能、炎症介质产生发挥抗炎作用。Aim To study the anti-inflammatory effects and mechanisms of SBM isolated from Seutellariae radix by high hydrostatic pressure. Methods The effect of SBM on ConA-induced splenocyte proliferation,LPS- induced PGE： production and protein expression of COX-2 were studied in vitro, and the therapeutic effects of SBM on carragineen-indueed paw oedema and acetic acid-induced twisting reaction were also investigated. Results SBM （ 15.63-250） mg · L^-1 significantly inhibited LPS-indueed PGE2 production and COX-2 enzyme aetivity in peritoneal maerophages in mice. SBM （125 mg· L^-1） also inhibited ConA-induced protein phosphorylation of p38 MAP kinase and LPS-induced COX-2 protein expression. SBM （10,20 mg~ kg-1） inhibited carragineen-induced paw oedema and acetic acid-induced twisting reaction. Conclusion SBM can inhibit inflammatory reaction. Its mechanisms may be related to the suppression of inflammatory medium production.

关 键 词：黄芩苷元 炎症 PGE2 COX-2 P38MAPK 

分 类 号：R-332[医药卫生] R284