腈水合酶产生菌Rhodococcuss p．HUST-1发酵条件的优化  

Optimization of Fermentation Conditions for Nitrile Hydratase Producing Microognism Rhodococcus sp. HUST-1

作　　者：朱婷婷[1,2] 秦智[1] 孙晓君[1] 蒋忠平[1] 

机构地区：[1]哈尔滨理工大学化学与环境工程学院,黑龙江哈尔滨150040 [2]新余高等专科学校太阳能科学与工程系,江西新余338000

出　　处：《江西师范大学学报（自然科学版）》2008年第6期667-670,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：黑龙江省自然科学基金(E2004-08);黑龙江省普通高等学校青年学术骨干支持计划(1153G008);哈尔滨市科技攻关项目(2004AA4CS049);哈尔滨市青年科学研究基金(2004AFQXJ041);新余高等专科学校重点校级课题(XJ0739)资助项目

摘　　要：Rhodococcus sp.HUST-1在Co2+的诱导下产腈水合酶,催化丙烯腈水合生成丙烯酰胺.对菌株HUST-1的产酶条件进行优化.研究表明,发酵过程中培养基中的碳氮源、诱导剂浓度以及培养条件影响腈水合酶的高效表达.在葡萄糖20 g/L、酵母粉10 g/L、Co2+0.08 mmol/L、酪氨酸0.2 g/L、pH7.0、培养温度28℃时,HUST-1所产腈水合酶总酶活达1 012.7 U,比优化前提高了47.1%.Rhodococcus sp. HUST-1 produces nitrile hydratase induced by Co^2＋ , as a catalyst which can synthesize acrylamide from hydration of acrylonitrile. Through optimizing fermentation conditions of the nitrile hydratase producing strain HUST-1, results showed that over-expression of nitrile hydratase was influenced by the concentration of carbon source, nitrogen source, inducer in culture medium and cultivated conditions. The optimal fermentation conditions were as the following： the concentration of glucose 20 g/L, yeast extract 10 g/L, Co^2＋ 0.08 mol/L,tyrosine 0.2 g/L, pH 7.0 and temperature 28 ℃. Under these conditions, enzyme activity could reach 1 012.7 U, which was 47.1% higher than before.

关 键 词：腈水合酶 发酵条件 丙烯腈 

分 类 号：TQ925[轻工技术与工程—发酵工程] TQ920.1