雌二醇诱致大鼠垂体催乳素瘤形成过程催乳素基因DNase　Ⅰ敏感位点增加  

The PRL Gene is Highly Sensitive to Digestion by DNase I in theChromation of Anterior Pituitary Tumors Induced by Estradiol

作　　者：狄安稞[1] 吴雪梅[1] 黄曼影[1] 单惠敏[1] 周远征[1] 

机构地区：[1]中国医学科学院基础医学研究所,中国协和医科大学基础医学院生理研究室

出　　处：《基础医学与临床》1998年第2期25-28,共4页Basic and Clinical Medicine

基　　金：国家自然科学资金;卫生部基金

摘　　要：ＤＨａｓｅⅠ敏感位点的存在，是染色质水平上基因活化调节的重要基础之一。为探讨Ｅ２诱发垂体ＰＲＬ瘤形成的表基因机制，本工作观察了Ｅ２诱致的垂体催乳素瘤催乳素基因ＤＮａｓｅⅠ敏感位点的改变。结果表明：Ｅ２诱致大鼠垂体前叶ＰＲＬ瘤细胞ＰＲＬ基因的ＤＮａｓｅⅠ敏感位点较正常大鼠增多，提示在Ｅ２诱发ＰＲＬ瘤形成过程中，ＰＲＬ基因处于活化状态。The present work determined whether there was. a change in the state of DNase I sites in the chromatin of anterior pituitary tumors induced -by estradiol in SD rat. by Southern blot hybridization. The results indicated that the PRL gene in the chromatin of anterior pituitary tumors induced by estradiol was more sensitive to digestion by DNase I than the control group. This suggested that PRL gene in the chromatin of anterior pituitary tumors induced by estradiol was in a more active state than the normal anterior pituitary.

关 键 词：雌二醇 垂体催乳素瘤 催乳素基因 DNASE 

分 类 号：R736[医药卫生—肿瘤] R73-3[医药卫生—临床医学]