鼻咽癌组织中P16基因缺失突变的分析  

Analysis on loss mutation of p16 gene in Nasopharyngeal Carcinoma

作　　者：宋丹[1] 何侠[1] 陈森清[1] 李金田[1] 郑必惠[1] 

机构地区：[1]江苏省肿瘤医院放疗科(南京),210009

出　　处：《齐齐哈尔医学院学报》2008年第24期2954-2956,共3页Journal of Qiqihar Medical University

基　　金：江苏省自然科学基金资助课题(BK2002136)

摘　　要：目的探讨p16基因缺失突变在鼻咽癌中的发生情况与临床意义。方法采用多重PCR分析法,对157例鼻咽癌标本及47例治疗后病理阴性组织标本P16基因突变情况进行了检测。结果鼻咽癌组织中P16基因第2外显子的缺失率高于疗后病理阴性组织,统计学上有显著差异性(P<0.000),P16基因第2外显子的缺失与鼻咽癌的临床分期、性别、年龄统计学上无显著差异(χ2值分别为1.726、0.582、0.196,P>0.05),P16基因第2外显子的缺失率不足3年生存组高于超过3年生存组,统计学上有显著差异性(χ2=14.12,P<0.000)。结论P16基因第2外显子缺失可能与鼻咽癌的发生发展预后有关,与临床分期、性别、年龄无关,可作为预后评价指标。Objective To investigate the status of p16 gene loss mutation in nasopharyngeal Carcinoma and clinical significance. Methods Total of 157 NPC cases and 47 cases after cure were detected for p16 gene loss mutations by using multiplex polymerase chain reaction（PCR） respectively. Results Loss of p16 gene exon 2 in tissues with nasopharyngeal carcinoma was higher than tissues after cure （P〈0. 000）. No significant relationship was found between loss p16 and the clinical stage, gender, age in NPC （P〉0.05）. The loss p16 were more frequent in tumors with （3 year survival than those with 〉 3 year survival （P 〈0. 000）. Conclusions Loss of p16 gene exon 2 in tissues with nasopharyngeal carcinoma may be involved in happen, development, prognosis of tumors , may not be involved in clinical stage, gender, age, and may be prognostic indicators in NPC.

关 键 词：鼻咽癌 P16 预后 多重PCR 缺失 突变 

分 类 号：R737.33[医药卫生—肿瘤] R739.63[医药卫生—临床医学]