激光诱变筛选呼吸缺陷型酵母及其发酵条件优化  

Selection of the respiration deficiency mutant yeast irritated by laser and optimization of the fermentation condition

作　　者：茅文俊[1] 刘真真[1] 朱虹[1] 朱融融[1] 孙晓宇[1] 姚思德[2] 汪世龙[1] 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]中国科学院上海应用物理研究所,上海201800

出　　处：《辐射研究与辐射工艺学报》2009年第1期1-4,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30570376);上海市教委科技创新项目(08ZZ21);基础重点项目(06JC14068)资助

摘　　要：本实验利用266nm激光作为诱变手段,对酿酒酵母YE0进行辐照诱变筛选呼吸缺陷型酵母,通过TTC(2,3,5-氯化三苯基四氮唑)筛选和气相色谱法酒精测定,最终得到一株高葡萄糖转化率呼吸缺陷型酵母JB7。对该突变株进行鉴定和发酵条件优化,结果表明,接种量15%,发酵温度32℃,葡萄糖初始浓度25%,发酵72h后,酒精产量体积分数可达12.3%,葡萄糖转化率达到52.9%,分别比出发菌株的酒精产量(12.0%)和葡萄糖转化率(49.6%)有所提高。高于正常酵母的糖原利用率,显示了呼吸缺陷型酵母良好的应用前景。A screen of respiration deficiency mutants of Saccharomyces Cerevisiae YEO induced by 266nm laser irradiation was studied. After the selective culture of TTC medium and determination of the alcohol yield by gas chromatographs, a respiration deficiency mutant JB7 with high glucose transformation efficiency was obtained. The results show that under the optimized fermentation conditions （ inoculation amount is 15%, glucose concentration is 25%, fermentation temperature is 32℃ and fermentation time is 72h） the alcoholic yield in volume fraction is 12.3% and the glucose transformation efficiency of JB7 is 52.9%, which are higher than that of the original strain YE0（glucose transformation efficiency is 49.6%, the alcoholic yield is 12.0%）. The high glucose transformation efficiency shows a good prospect of application of the respiration deficiency yeast.

关 键 词：激光 酿酒酵母 发酵 

分 类 号：Q345.1[生物学—遗传学] Q631