低温碱性脂肪酶产生菌的筛选及产酶培养基的优化  

Screening and Optimization of Fermentation Medium of Bacterium Producing Cold-Adapted and Alkaline Lipase

作　　者：刘瑞娟[1] 王海宽[1] 路福平[1] 戚薇[1] 

机构地区：[1]工业微生物教育部重点实验室天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2009年第1期6-10,共5页Journal of Tianjin University of Science & Technology

基　　金：天津市应用基础研究计划面上项目资助(07JCYBJC07900)

摘　　要：以橄榄油为唯一碳源,从渤海湾盐碱地被油污染的土样中分离筛选出1株产低温碱性脂肪酶菌株34-5,初步酶学性质研究表明,该菌所产脂肪酶的最适温度为25,℃,最适pH为9.6.该菌的16S rDNA基因序列与伯克霍尔德氏菌(Burkholderia)的同源性为99%.摇瓶实验表明,该菌株最适产酶培养基为(g/L):淀粉10,黄豆饼粉20,玉米浆20,K2HPO4 1,聚乙烯醇大豆油乳化液20.其最高酶活为6.87,U/mL.A bacterium which produces cold-adapted alkaline lipase was isolated from soil on a selective medium that contained olive oil as the only source of carbon. The soil was collected from bay of Bohai. The optimal temperature and pH of the lipase were 25℃ and pH 9.6, respectively. The sequence of 16S rDNA of strain 34-5 showed 99% homology to Burkholderia. The optimal medium for lipase production were determined as follows （g/L） ：starch 10, bean flour 20, corn syrup 20, K2HPO4 1, emulsioned bean oil 20. The alkaline lipase activity reached maximal level of 6.87 U/mL.

关 键 词：低温脂肪酶 碱性脂肪酶 筛选 

分 类 号：Q933[生物学—微生物学]