17α-甲基睾丸素C1,2位高效生物脱氢菌种的选育  

Breeding of Strain for High-Level C1,2-Dehydrogenation of 17α-methyl-testosterone

作　　者：陈莹[1] 李静文[1] 别松涛[1] 

机构地区：[1]工业微生物教育部重点实验室天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2009年第1期22-25,共4页Journal of Tianjin University of Science & Technology

基　　金：国家科技基础条件平台资助项目(2005DKA21204-10)

摘　　要：为利用简单节杆菌生物催化17α-甲基睾丸素C1,2位脱氢制备去氢甲基睾丸素,采用N+离子注入与Cs137-γ射线辐照两种方法进行菌种诱变,选育适宜于17α-甲基睾丸素C12,位脱氢的高转化菌株.结果表明,从16株简单节杆菌筛选出的TCCC11042,依次经N+离子注入与Cs137-γ射线辐照处理,通过甲基睾丸素为底物的摇瓶筛选,选育得到正变株F2-20,该菌株对17α-甲基睾丸素脱氢转化率达到60.8%,比出发菌株TCCC11042的转化率提高了12.8%.经群体传代方式考察,诱变株具有良好的遗传稳定性.Two mutation methods of N^＋ ion implantation and Cs^137-γ-ray irradiation were used to treat Arthrobacter simplex,and to obtain the mutant with high C1,2-dehydrogenation activity from 17α-methyl-testosterone to dehydrogenmethyl-testosterone. The original A.simplex TCCC 11042 ,which was screened from 16 strains of A.simplex based on the result of dehydrogenation of 17α-methyl-testosterone in flask,was mutated by N^＋ ion implantation and Cs^137-γ-ray irradiation successively. The results show that one positive mutant F2-20 from TCCC11042 was bred, and the mutant had a high bioconversion ratio of 60.8% from 17α-methyl-testosterone to dehydrogen-methyl-testosterone,which was higher 12.8% than the original strain TCCC11042 with only 48%. The mutant kept a stable heredity with higher C1,2-dehydrogenation of 17α- methyl-testosterone after colony successive inoculation test.

关 键 词：简单节杆菌 17α-甲基睾丸素 C12 位脱氢 离子注入 Γ射线辐照 

分 类 号：Q93-31[生物学—微生物学]