应用双特异分子探针技术定性定量检测圆海链藻(Thalassiosira rotula)  

DETECTION AND QUANTIFICATION OF THALASSIOSIRA ROTULA WITH DOUBLE SPECIFIC PROBE ASSAY

作　　者：李秀芹[1] 于志刚[2] 甄毓[2] 蔡青松[3] 米铁柱[4] 李荣秀[5] 

机构地区：[1]中国海洋大学生命科学与技术学部,青岛266003 [2]中国海洋大学化学化工学院,青岛266003 [3]中国科学院上海生命科学研究院,上海200031 [4]中国海洋大学环境科学与工程学院,青岛266003 [5]上海交通大学生命科学技术学院,上海200030

出　　处：《海洋与湖沼》2009年第1期12-18,共7页Oceanologia Et Limnologia Sinica

基　　金：国家高技术研究发展计划(863计划)资助项目;2001AA635090号;国家高技术研究发展计划(863计划)资助项目;2003AA635010号;自然科学基金资助项目;40406028号

摘　　要：设计了一套圆海链藻(Thalassiosira rotula)特异性探针,运用双特异分子探针技术,对圆海链藻(Thalassiosira rotula)进行了定性定量检测。结果表明,本实验中设计的一套探针与其它十几种藻无交叉反应,具有种特异性;细胞裂解液直接杂交检测优于提纯RNA样品检测;对自然样品做了初步检测,发现天然海水中的其它浮游生物对该检测方法影响很小。A double specific probe assay was applied to detect and quantify Thalassiosira rotula, a common species of harmful algae bloom, in which a set of oligonucleotide probes targeting 18S rRNA was used. The probes are species-specific, without obvious incident cross-reactivity with other species. Results of detection on the same number of cells to crude cell lysate and extracted total RNA were compared, showing that the former was better. A preliminary test was made on natural samples, and the noise produced by complex field background was minimal.

关 键 词：圆海链藻 双特异分子探针技术 赤潮藻检测 

分 类 号：X172[环境科学与工程—环境科学]