微量含硒蛋白检测技术进展  

Progress in the Detection of Selenium-Containing Trace Proteins

作　　者：刘琼[1] 彭珍[1] 梁雪莹[1] 

机构地区：[1]深圳大学生命科学学院,广东深圳518060

出　　处：《光谱学与光谱分析》2009年第2期530-535,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30570420;20637010)资助

摘　　要：生物必需微量元素硒在体内主要通过硒蛋白发挥作用,因此微量含硒蛋白的高灵敏检测和识别是验证生物信息学所预测的硒蛋白和开展硒蛋白组学研究的重要手段,也是硒蛋白功能研究的先决条件。文章重点介绍了近年迅速发展的激光剥蚀电感耦合等离子体质谱技术原理及其对凝胶电泳分离后蛋白质硒的检测,简要综述了凝胶电泳分离后其他质谱技术在含硒蛋白分析鉴定中的应用,包括毛细管高效液相色谱/纳升高效液相色谱-电感耦合等离子体质谱对含硒多肽的分离检测、基质辅助激光解吸离子化-飞行时间-质谱对含硒蛋白的识别、以及纳升高效液相色谱-电喷雾-串联质谱对含硒蛋白的鉴定等内容,指出了微量含硒蛋白检测中存在的问题和发展方向。Essential trace element selenium （Se） exerts its biological functions in vivo mainly through selenoproteins. Thus highly sensitive detection of Se-containing trace proteins is the key step toward the confirmation of selenoproteins predicted bioinformatically from genomes or separated electrophoretically in the study of selenoproteomics. This review mainly describes the principle of a new technique of laser ablation-inductively coupled plasma-mass spectrometry （LA-ICP-MS）, which has been fast developed in recent years, and its application to the detection of Se in proteins separated by gel eleetrophoresis. The paper also summarizes the progress of mass spectrometry in the identification and charact.erization of Se-containing proteins after gel electro- phoresis, including the detection of Se-containing peptides by ICP-MS hyphenated with either capillary high performance liquid chromatography or nano HPLC, the identification of Se-containing proteins by matrix-assisted laser desorpfion/ionization time-of-flight mass spectrometry, and the characterization and confirmation of selenoproteins by nano HPLC-electrospray tandem mass spectrometry. Problems presented in those methods are also discussed in this paper for further study.

关 键 词：硒蛋白 激光剥蚀 电感偶合等离子体 质谱 高效液相色谱 

分 类 号：O657.3[理学—分析化学]