鞘氨醇单胞菌TP-3合成新型生物聚合物Ss的发酵条件优化  

Optimization of Culture Condition for Novel Biopolymer Ss Production by Sphingomonas sp. Strain TP-3

作　　者：黄海东[1,2] 王薇[1] 马挺[1] 张禹 张国沛 李国强[1] 梁凤来[1] 刘如林[1] 

机构地区：[1]南开大学生命科学学院分子微生物学与技术教育部重点实验室,天津300071 [2]天津农学院农学系,天津300384 [3]鑫合生物化工有限公司,河北新河055650

出　　处：《微生物学通报》2009年第2期155-159,共5页Microbiology China

基　　金：国家自然科学基金项目(No.50674058)资助

摘　　要：鞘氨醇单胞菌(Sphingomonas sp.)TP-3能合成一种具有增稠性、假塑性、成凝胶特性和乳化性能的新型生物聚合物Ss。运用单因素实验和均匀设计法对菌株TP-3合成聚合物Ss的发酵条件进行优化,实验结果表明,培养基组成为葡萄糖41.2g/L,豆饼粉2.0g/L,NaCl 0.85g/L,K2HPO4 1.46g/L,MgSO4 0.12g/L,MnCl2 0.0075g/L,FeSO4 0.002g/L,初始pH为7.0,在27℃,180r/min的条件下摇床培养60h,聚合物Ss的产量达到21.5g/L。该聚合物生产成本低,在油田开发中极具应用前景。Sphingomonas sp. TP-3 synthesizes a type of novel biopolymer Ss with properties of thickening, pseudoplastic, gelling and emulsification. Both single factor experiments and uniform design were employed in this study to optimize fermentation conditions of strain TP-3. The optimal fermentation paramenters in shaking flask were as follow： glucose 41.2 g/L, soybean meal 2.0 g/L, NaCl 0.85 g/L, K2HPO4 1.46 g/L, MgSO4 0.12 g/L, MnCl2 0.0075 g/L, FeSO4 0.002 g/L, initial pH 7.0, culture temperature 27℃, rotating speed 180 r/min. The yield of biopolymer Ss reached 21.5 g/L. As a low-cost biopolymer, Ss has wide pros- pect for application in oil recovery.

关 键 词：鞘氨醇单胞菌 生物聚合物 发酵条件 均匀设计 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]