293细胞检测致癌物的应用研究  

Application of 293 cells in vitro detection of carcinogens

作　　者：赵蕊[1] 张磊[2] 钟儒刚[1] 周玲[2] 曾毅[2] 

机构地区：[1]北京工业大学生命科学与生物工程学院,100022 [2]中国疾病预防控制中心病毒病所国家重点实验室肿瘤病毒组

出　　处：《中华实验和临床病毒学杂志》2009年第1期47-49,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家863资助项目（2006BA119B03）

摘　　要：目的探讨应用293细胞对致癌物进行细胞转化及致瘤性等研究，为检测可疑致癌物提供实验依据。方法应用微囊藻毒素LR（Microcystin LR，MC．LR）对293细胞进行转化培养，取转化后的细胞进行软琼脂克隆实验、血清依赖性实验以及免疫缺陷小鼠体内致瘤实验来验证MC-LR转化的293细胞的致癌性。结果293细胞在MC-LR的作用下，逐渐表现出转化细胞的特性：血清依赖程度显著降低，在软琼脂培养基中锚着独立生长并形成细胞集落，在SCID小鼠体内形成浸润性肿瘤组织。结论293细胞易于培养并对环境致癌物敏感，可用于可疑致癌物的检测。Objective To investigate the application of 293 cells to detect suspected carcinogens and provide experimental evidence by using in vitro cell transformation assay and tumorigenicity study. Methods The transformation systems of cells cultured in vitro have been adopted to clarify the tumor promotive activity of Microcystin LR （MC-LR）. The malignant transformation of 293 ceils induced by MC-LR is tested by several methods including clone forming in soft agarose, serum requirement assay and tumor forming in mice to define the promotive activity of 293 cells. Results 293 cell acted like tumor cells after induced by MC-LR： serum dependence decreased, anchorage independence growth in soft agarose and formed cell clones, malignant tumors appeared in SCID mice. Conclusion 293 cells were easy to culture and sensitive to environmental carcinogens so that can be used in detection of suspicious carcinogens.

关 键 词：细胞系 微囊藻毒素 细胞转化 肿瘤 致癌物 

分 类 号：R73-3[医药卫生—肿瘤]