高压脉冲电场对脂肪氧化酶二级和三级构象的影响效果  

Effects of High Pulsed Electric Field on the Secondary and Tertiary Structure of Lipoxygenase

作　　者：钟葵[1,2] 胡小松[2] 吴继红[2] 陈芳[2] 廖小军[2] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070 [2]教育部果蔬加工工程中心中国农业大学,北京100083

出　　处：《光谱学与光谱分析》2009年第3期765-768,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30371003);国家科技支撑计划项目(2006BAD05A02)资助

摘　　要：应用圆二色谱和荧光光谱研究了高压脉冲电场(PEF)对脂肪氧化酶(LOX)的二级和三级构象的影响效果。研究结果表明PEF破坏了LOX二级和三级结构。PEF处理后,LOX的CD光谱中两个特征负峰值显著降低,二级结构中α-螺旋含量显著降低(p<0.05),并且LOX中α-螺旋含量和电场强度之间存在良好线性关系。PEF处理后LOX发射光谱中特征峰(337和583 nm)的荧光强度显著增大(p<0.05),特征峰相对荧光强度和电场强度之间存在良好线性关系。结果表明PEF酶活钝化跟酶二级和三级结构的破坏有关,为PEF钝酶机理研究提供理论基础。The effect of pulsed electric field （PEF） on the secondary and tertiary structure of lipoxygenase （LOX） in a buffer solution was analyzed using far UV-circular dichroism （CD） and fluorescence spectrophotometry, respectively. The secondary and tertiary structure of LOX changed after PEF treatment. The CD spectra of LOX also changed, with the intensity of two negative peaks and the content of α-helix significantly decreased （p〈0. 05）. The decrease in α-helix content in LOX showed a good linear correlation with the electric field strength. The fluorescence intensity of LOX increased, and the relative fluorescence intensity of the two characteristic peaks of LOX emission spectra at 337 nm and 583 nm also showed a good linear correlation with the electric field strength. These results showed that the activity inactivation of LOX may be due to the alteration in secondary structure, and both had a good relation.

关 键 词：脉冲电场 脂肪氧化酶 构象 圆二色谱 荧光光谱 

分 类 号：O657.3[理学—分析化学] Q683[理学—化学]