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作 者:方清明[1,2] 李学明[1,2] 李健学[1,2] 梁曙光[1,2] 刘鸿君[1,2] 徐坤[1,2]
机构地区:[1]中国预防医学科学院环境卫生与卫生工程研究所 [2]中国科学院生态环境研究中心
出 处:《卫生研究》1998年第1期6-8,共3页Journal of Hygiene Research
基 金:国家自然科学基金;卫生部医学研究基金
摘 要:应用聚合酶链式反应(PCR)-单链构象多态性(SSCP)-DNA序列分析法,对40例肺癌样品中K-ras基因突变进行检测。PCR-SSCP银染分析,突变检出率为30%(12/40),发现于肺腺癌的K-ras基因突变率为44%(12/27);DNA序列分析,12例筛选突变阳性样品中的11例检出了突变位点,90%的突变发生于12密码子,其突变谱以G→T颠换(占40%)和G→A转换(占60%)为主。本研究结果显示SSCP银染分析对大量样品进行阳性筛选有很好的应用价值,PCR-SSCP-DNA序列分析法是检测基因突变的有效手段。 ras oncogene mutations were detected with PCR SSCP DNA direct sequencing technique in 40 cases of lung cancer.The result of PCR SSCP silver staining indicated that the mutational rate was 30%(12/40),all mutations were observed in lung adenocarcinoma and its mutational rate was 44%(12/27).The result of DNA direct sequencing showed that 11 of the 12 positive samples screened by PCR SSCP had mutation and 90% of K ras mutations were in codon 12.The mutation was mainly G→T transversion and G→A transition.The study suggested that SSCP silver staining analysis is very useful in screening large amount of samples simultaneously and PCR SSCP DNA direct sequencing method is quite efficient for the detection of oncogene mutation.
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