蛋白质组学在脑缺血预适应研究中的应用  

Application of proteomics in the studies of cerebral ischemic preconditioning

作　　者：刘媚[1] 陈晓红[1] 

机构地区：[1]中山大学第三附属医院神经内科,广州510080

出　　处：《国际脑血管病杂志》2009年第1期63-66,共4页International Journal of Cerebrovascular Diseases

基　　金：国家自然科学基金（30870849）

摘　　要：蛋白质组是由一个细胞、一个组织或一个机体的基因组所表达的全部相应蛋白质。蛋白质组学是以蛋白质组为研究对象，从蛋白质整体水平认识生命活动规律的科学，也是定量检测细胞或组织的一个或多个蛋白群或蛋白质组的科学方法。脑缺血预适应是近几十年来神经科学领域的研究热点之一，其神经保护机制与缺血后蛋白表达水平改变有关，因此，蛋白质组学在其机制研究中显得越来越重要。文章主要介绍蛋白质组学的主要技术及其在脑缺血预适应研究中的应用。Proteome is defined as all proteins expressed in a cell, a tissue or an organism. Proteomics, a science of understanding the life activity law at the whole protein level, takes proteome as a research object; it is also a scientific method for quantitative detection of one or more protein populations or proteomes in a cell or tissue. Cerebral ischemic preconditioning has been one of the research hotspots in the field of neuroscience for decades. Its neuroprotective mechanism is associated with the changes of protein expression levels after cerebral ischemia. Therefore, proteomics has become more and more important in its mechanism study. This article mainly presents the major techniques of proteomics and their application in the studies of cerebral ischemic preconditioning.

关 键 词：蛋白质组学 脑缺血 预适应 双向电泳 质谱 

分 类 号：R741[医药卫生—神经病学与精神病学]