葡萄酒中痕量木塞污染物的顶空固相微萃取-气相色谱串联质谱法检测  

Determination of Cork Taint(2,4,6-Trichloroanisole) in Wine by Headspace Solid-phase Microextraction-Gas Chromatography Coupled to Tandem Mass Spectrometry

作　　者：邓晓军[1] 郭德华[1] 李波[1] 朱坚[1] 张龙[1] 生茂强[2] 陈舜胜[2] 

机构地区：[1]上海出入境检验检疫局,上海200135 [2]上海水产大学食品学院,上海200090

出　　处：《分析测试学报》2008年第12期1375-1378,共4页Journal of Instrumental Analysis

摘　　要：建立了顶空固相微萃取-气相色谱串联质谱检测葡萄酒中主要的痕量木塞污染物——2,4,6-三氯苯甲醚（TCA）的方法。通过优化萃取时间、温度、盐浓度、pH值等固相微萃取处理条件,采用2,4,6-三氯甲苯（TCT）为内标进行定量,气相色谱离子阱质谱法测定。选取TCA母离子和子离子分别为m/z210和m/z195,TCT的母离子和子离子为m/z195和m/z159。方法的定量下限（LOQ）为2.0 ng/L,回收率为71%-98%。该法操作简单、快速,适用于葡萄酒中痕量TCA残留的快速检测。A method for determination of trace level of 2, 4, 6-trichloroanisole （TCA） in wine has been presented by using headspace solid-please microextraction -gas chromatography coupled to tandem mass spectrometry（SPME -GC -MS/MS）. The extraction parameters such as time, temperature, salt concentration, and sample volume were optimized. The analysis was performed by GC - MS/MS using 2, 4, 6-trichlorotoluene（TCT） as an internal standard. The m/z 210 and 195 were selected as the parent ions and m/z 195 and 159 as the daughter ion for TCA and TCT, respectively. The recoveries of fortified wine at three levels ranged from 71% to 98%. LOQ of the method was 2.0 ng/L. The method is simple, rapid, and suitable for the determination of TCA residue in wine.

关 键 词：固相微萃取 木塞污染 2 4 6-三氯苯甲醚 葡萄酒 气相色谱串联质谱 

分 类 号：O657.63[理学—分析化学] TS262.6[理学—化学]