槲皮素-H2O2-硼砂荧光光度法测定方法的研究  

STUDIES ON FLUORIMETRIC METHOD FOR QCT,H_2O_2 AND BORAX

作　　者：梅洪睿[1] 云霞[1] 王安良[1] 杨红[1] 樊志鹏 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]辽宁省凤城市第一中学,辽宁丹东118100

出　　处：《食品研究与开发》2009年第3期25-28,共4页Food Research and Development

摘　　要：研究槲皮素被过氧化氢氧化后的产物在硼砂溶液中的荧光性质,确定最佳条件。在激发波长和发射波长分别为305nm和355nm时,发射荧光强度和槲皮素的浓度在0mol/L～3.2×10-5mol/L的范围内成线性关系,线性方程为F=2.5+1.64196C(10-6mol/L),相关系数r=0.9997。将此方法应用在菜芙蓉样品中的槲皮素含量的测定,精确度可达到1.435%,回收率为97.1%～103.7%。It was found that quercetin oxidized by hydrogen dioxide emits strong fluorescence in borax buffer. Under the optimum experimental conditions,when the excitation and emission wavelengths were 305 nm and 355 nm, respectively; and the linear range was 0 mol/L-3.2×10^-5 mol/L for QCT,the linear equation was F= 2.5＋ 1.641 96 C （10^-6 mol/L）, and the related coefficient was r=0.999 7. If the method was applied to the determination of QCT in the samples of Hibiscus manihot, RSD was 1.435 %, recoveries obtained were in the range 97.1%-103.7 %.

关 键 词：槲皮素 过氧化氢 硼砂 荧光光度法 菜芙蓉 

分 类 号：R284[医药卫生—中药学]