VEGF反义寡核苷酸与顺铂联合治疗抑制裸鼠骨肉瘤的生长  

VEGF Antisense Oligonucleotides in Combination with DDP Inhibited the Growth of Osteosarcoma Nude Model

作　　者：方松清[1] 曾谷清[1] 

机构地区：[1]南华大学医学院外总手术学教研室,湖南衡阳421001

出　　处：《南华大学学报（医学版）》2009年第1期36-38,共3页Journal of Nanhua University(Medical Edition)

基　　金：湖南省卫生厅课题资助项目(4-04-WS-B2004-089)

摘　　要：目的探讨血管内皮生长因子(VEGF)反义寡核苷酸和顺铂单用或联用对裸鼠骨肉瘤生长的抑制作用。方法制备BALC/C裸鼠皮下骨肉瘤模型24只,随机分为4组:VEGF反义寡核苷酸治疗(ASODN)组、顺铂治疗(DDP)组、联合治疗(ASODN+DDP)组和对照组,每组6只。接种MG-63人成骨肉瘤细胞后24 h内采用瘤内注射和腹腔给药的方式,以VEGF反义寡核苷酸或(和)顺铂进行治疗;观察各组裸鼠肿瘤的生长情况、裸鼠的一般情况与生存期。断颈处死裸鼠,测量肿瘤体积大小,称量肿瘤的重量。结果VEGF-ASODN治疗组、DDP治疗组和联合治疗组裸鼠骨肉瘤的生长均受到不同程度抑制,抑瘤率分别为44.56%、53.69%和86.80%,以联合治疗组的疗效最佳。结论VEGF反义寡核苷酸与顺铂联用比单用顺铂可更好地抑制骨肉瘤的生长,两者联用可发挥协同抗瘤效应。Objective To study the inhibitory effect of VEGF antisense oligonucleotides or/and DDP on the growth of Osteosarcoma Nude model. Methods The Osteosarcoma cells were cultured and implanted subcutaneously into 24 nude mice model which were subsequently divided into 4 groups ： VEGF ASODN group, DDP group, combined group and control group. The different treatments were given respectively at 24 hours after cell inoculation. The weight and volume of subcutaneous tumors were measured. Result The growth inhibitory rate in VEGF - ASODN group was 44.56% ,53.69% in the DDP group,and 86.80% in the combined group. The combined group which was treated with VEGF -ASODN and DDP showed more effective inhibition than the VEGF - ASODN group or the DDP group respectively. Conclusion The growth of Osteosarcoma nude model can be inhibited by VEGF antisense oligonueleotides and DDP respectively. Better inhibitory effect can be obtained if VEGF - ASODN and DDP are combined in use.

关 键 词：血管内皮生长因子 顺铂 反义寡核苷酸 骨肉瘤 

分 类 号：R738.7[医药卫生—肿瘤]