响应面法优化桦褐孔菌胞外多糖发酵条件  

Optimization of the Polysaccharide Accumulation by Inonotus Obliquus in Submerged Fermentation

作　　者：张泽生[1] 史佳宁[1] 张婕[1] 张建平[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《现代食品科技》2009年第3期298-301,295,共5页Modern Food Science and Technology

摘　　要：为了获得更多的桦褐孔菌胞外多糖,以单因素摇瓶实验和响应面分析对药用真菌桦褐孔菌培养基进行了优化,确定了优化培养基(g/L):玉米糖化液21.1、可溶性淀粉22.3、酵母浸粉5.1、蛋白胨4.1、KH2PO42、MgSO40.5,起始pH为6.0。在优化条件下,研究了0～12d,桦褐孔菌发酵pH、还原糖、胞外粗多糖、生物量的变化情况。摇瓶发酵结果表明,整个过程中pH变化不明显,生物量先增后趋于稳定,于6d达最大值11.35g/L,胞外粗多糖先降后升又降,在7d达最大3.85g/L。For polysaccharide production, both the one-factor-at-a-time and SAS methods were applied to the optimization of submerged fermentation by Inonotus obliquus. The best components of the medium were determined as following： corn syrup 2.11%, soluble starch 2.23%, yeast powder 0.51%, peptone 0.41%, KHzPO4 0.2%, and magnesium sulfate 0.05%. Using the optimum media, the culture pH, reducing sugar, polysacchafide and mycelia biomass oflnonotus obliquus in 12 days were discussed. Results showed that, during the fermentation, constant pH value was found. The mycelia biomasses was increased firstly and then reached the maximum value of 1.35 g/L at 6 days, while the crude polysaccharide content was increased firstly and then decreased later with its highest value of 3.85 at 7days.

关 键 词：桦褐孔菌 胞外多糖 响应面优化 

分 类 号：S567.39[农业科学—中草药栽培]