放线菌CCTCC M207210所产青霉抑制物的稳定性及应用  

Stability of Antifungal Substance Produced by Actinomycete CCTCC M207210 and Its Application

作　　者：朱从会[1] 师俊玲[1] 杨保伟[1] 孙卉[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《中国农业科学》2009年第2期636-641,共6页Scientia Agricultura Sinica

基　　金：国家科技支撑计划(2006BAK02A24);西北农林科技大学青年学术骨干支持计划

摘　　要：【目的】明确放线菌CCTCCM207210发酵液的青霉抑制活性在不同处理条件下的稳定性,评价其在苹果贮藏与果汁加工中的应用潜力。【方法】将无菌体发酵液经热、酸、碱、紫外线等处理不同时间后,用滤纸片法在琼脂平板培养基上检验发酵液对产展青霉素扩展青霉SP2204、SP2205生长的抑制活性;在接种扩展青霉的苹果和苹果汁上喷涂或加入无菌体发酵液,分别培养12和7d后,测定苹果的病斑直径和果汁中展青霉素产量。【结果】加热、酸碱处理和紫外线照射都会破坏发酵液对扩展青霉生长的抑制活性:经100℃保温处理60min,或紫外线照射(30W,245nm,15cm)90min,发酵液的抑菌活性完全丧失;发酵液的抑菌活性在发酵液原始pH(5.7)下最稳定,偏碱性条件的稳定性稍高于偏酸性条件。接种扩展青霉120h后,在苹果表面喷涂无菌体发酵液可使苹果病斑直径的扩展速度降低25%;在果汁中加入40%(v/v)的无菌体发酵液,可使展青霉素产量降低90%。【结论】放线菌CCTCCM207210发酵液对扩展青霉的抑菌活性具有一定的热、酸碱和紫外线耐受能力,在苹果贮藏和果汁加工中抑制扩展青霉素的生长与产毒方面有一定应用潜力。[ Objective ] The paper aimed at investigating the stability and potential application of the antifungal substance in the cell-free liquid culture of Actinomycete CCTCC M207210. [ Method ] The antifungal ability of the cell-free liquid culture of Actinoraycete CCTCC M207210 was tested on agar media after pretreatment of heat, acid, alkali, and ultraviolet radiation. The cell-free liquid culture was added into apple juice medium and sprayed on apple surface after inoculation of patulin-producing Penicillium expansum SP2204 and SP2205. After a period of cultivation of 12 d, 7 d respectively, the diameter of rotten spot in apple was measured and patulin production in the apple juice culture was tested to evaluate the inhibitory ability of the cell-free culture in practical application. [Result] Heating, acidic and alkaline treatment, and ultraviolet radiation could destroy, to some extent, the antifungal activity of the cell-free liquid culture of Actinomycete CCTCC M207210. The antifungal activity was completely destroyed when the cell-free liquid culture was pretreated at 100℃ for 60 min or subjected to ultraviolet radiation （30 W, 245 nm, 15 cm distance） for 90 min. The liquid culture showed the most stable antifungal activity at the original pH （5.7） and more stable at a little alkaline pH than acidic condition. Extension rate of the rotten spots was reduced by 25% when the liquid culture was sprayed on the apple surface 120 h after the inoculation of P. expansum. Patulin production was reduced by 90% when the liquid culture was added in the apple juice cultivation of P.expansum in ratio of 40% （v/v）. [Conclusion] The antifungal substance in the cell-free liquid culture of Actinomycete CCTCC M207210 showed high resistibility to heat, acidic and alkaline conditions, and ultraviolet radiation, and had great potential in application of inhibiting the growth and patulin production of P. expansum for apple storage and apple juice production.

关 键 词：苹果 展青霉素 放线菌CCTCC M207210 抑菌 稳定性 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]