反刍动物饲料中乳源性成分与牛羊肉骨粉的区分  

Distinction and Identification of Milk Powder and Bovine or Ovine Meat and Bone Meal in Ruminant Feedstuffs

作　　者：胡菡[1] 王加启[1] 卜登攀[1] 魏宏阳[1,2] 叶巧燕[2] 刘开朗[1] 周凌云[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193 [2]农业部奶及奶制品质量监督检验测试中心北京,北京100193

出　　处：《农业生物技术学报》2009年第1期67-72,共6页Journal of Agricultural Biotechnology

基　　金：“十一五”国家奶业科技支撑计划(No.2006BAD12B08和2006BAD04A03)资助

摘　　要：利用奶粉的溶解性,通过水和0.5%次氯酸钠溶液洗涤混有乳源性成分和牛、羊肉骨粉的饲料样品,去除饲料中的乳粉成分后,再使用16S rDNA PCR方法进行动物源性检测。结果表明,实验所建立的方法能够完全区分饲料中的乳粉与肉骨粉。当乳粉含量分别为25%、50%和75%时,混合饲料中牛、羊肉骨粉的检出限分别为2%、0.5%和0.1%。此方法操作简单,容易掌握,可用于鉴别反刍动物饲料中非乳源性成分的牛、羊源性成分。A method was established to distinguish milk powder and bovine or ovine meat and bone meal in feedstuffs which were all ruminant-derived. Milk powder in feedstuffs was washed and removed by water and 0.5% sodium hypochlorite solution, subsequently, the ruminant-derived materials were identified using PCR according to 16S rDNA sequence. The detected limitation of bovine or ovine in feedstuffs with 25%, 50% and 75% milk powder concentrations were 2%, 0.5% and 0.1%, respectively. Taken altogether, this method is a simple and easily grasped way for identification and distinction of the bovine or ovine-derived meat and bone meal and milk powder in feedstuffs.

关 键 词：反刍动物饲料 奶粉 肉骨粉 16S RDNA PCR 

分 类 号：S188[农业科学—农业基础科学]