重组工业红色糖多孢菌优化红霉素发酵液组分  

Improvement of erythromycin A purity and production in a recombinant industrial Saccharopolyspora erythraea strain

作　　者：李学志[1] 钱江潮[1] 陈云[1] 杭海峰[1] 储炬[1] 庄英萍[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《中国抗生素杂志》2009年第3期152-155,180,共5页Chinese Journal of Antibiotics

基　　金：863工业生物技术重点项目(2006AA020304);上海市科委重点项目(074319115-3)

摘　　要：本文对代谢工程改造红色糖多孢菌ZL1004进行了摇瓶发酵试验,摇瓶发酵结束时重组菌红霉素生物效价与工业生产菌HL3168相比提高20%,红霉素A组分比例由84%提高到94%,红霉素A浓度3.98mg/ml,相比出发菌3.25mg/ml提高22%,重组菌中红霉素B、C与出发菌相比分别减少了51%和58%,实现了红霉素组分的优化。对重组菌摇瓶发酵过程生理代谢进行了初步的研究,表明重组菌生理代谢特性与原出发菌没有明显差异。对重组菌发酵过程中红霉素组分的变化规律进行分析,发现红霉素A、B、C组分的变化规律与eryK和eryG基因转录水平能较好地对应。In this paper, the performance of recombinant industrial Saccharopolyspora erythraea strain ZL1004 was studied in shake flasks. The ratio of Er-A was increased from 84% to 94% with a final concentration of 3.98mg/ml which was 22% higher than that of the original HL3168 strain （3.25mg/ml）. Furthermore, the concentrations of Er-B and Er-C were decreased significantly by 51% and 58% respectively. The study on the primary physiological characteristics of recombinant strain ZL1004 showed no apparent difference in glucose, nitrogen, phosphoric consumption rate in comparison with HL3168 strain. The analysis of Er-A, Er-B and Er-C ratios of both ZL1004 and HL3168 in the culture broth during the fermentation process showed a good correlation with the result of transcription level of the eryK and eryG genes in these strains.

关 键 词：红霉素 组分 重组菌 发酵 

分 类 号：Q784[生物学—分子生物学]