高效液相色谱同时检测保健食品中五种违禁的性功能药物成分  

Simultaneous determination of five species of contraband medicine component to enhance sexual function in health food by HPLC

作　　者：万春花[1] 魏强[2] 龙洲雄[1] 胡海山[1] 鄢兵[1] 范青生[2] 

机构地区：[1]江西省分析测试研究所,南昌330029 [2]南昌大学食品科学和生物技术国家重点实验室,中德联合研究院,南昌330047

出　　处：《食品科技》2009年第3期275-279,共5页Food Science and Technology

摘　　要：目的:建立一种同时测定保健食品中5种常见违禁添加的增强性功能药物成分的反相液相色谱方法。用二极管阵列检测器检测,利用色谱峰保留时间和物质的紫外吸收光谱综合定性,外标法定量。方法:采用反相高效液相色谱法实现5种成分完全分离。Agilent C18 4.6mm×250mm色谱柱,柱温30℃,流动相为乙腈/0.02%磷酸,梯度洗脱,流速1.0mL/min,检测波长为225.0nm。结果:5种违禁性功能药物成分在4.0～200.0μg/mL浓度范围内工作曲线线性关系良好,相关系数r≥0.9995,精密度相对标准偏差≤2.53%(n=6),平均回收率>94.0%,最低检出限≤56ng/mL(信噪比≥3)。结论:该方法简便快速,结果准确可靠,可用于保健食品中常见添加的性功能药物成分的测定。Objective： To establish a practical method of RP-HPLC for the simultaneous determination of five kinds of contraband medicine component to enhance sexual function in health food. Photodiode array detector was used, basing on chromatogram retention time and photodiode array spectrum for qualitative analysis and external standard method with peak area for quantitative analysis. Methods： The five components could be separated completely on a Agilent C18 column（4.6 mm×250 mm） and then detected at 225.0 nm, using acetonitdle and 0.02% phosphoric acid with gradient elution as the mobile phase at flow rate 1.0 mL/min, and the column temperature was 30 ℃. Results： The linear range of the calibration curve of five components were 4.0-200.0μg/ mL（r≥0.9995）, RSD≤2.53%（n=6）, The rates of average recovery were more than 94%, The detection limits of five components were less than or equal to 56 ng/mL（S/N ≥ 3）. Conclusion： The method is simple, rapid, accurate and reliable that can be used to determine frequent contraband ingredient to enhance sexual function in health food.

关 键 词：高效液相色谱 保健食品 违禁药物成分 测定 

分 类 号：TS207.5[轻工技术与工程—食品科学]