高效液相色谱法测定玉米中玉米赤霉烯酮  

Determination of Zearalenone in Corn by High Performance Liquid Chromatography

作　　者：罗小虎[1] 包清彬[1] 杨潇[1] 孔凌[1] 

机构地区：[1]西华大学生物工程学院,四川成都610039

出　　处：《粮食与饲料工业》2009年第3期48-49,共2页Cereal & Feed Industry

基　　金：国家粮食局"十一五"科技支撑计划资助项目(2006BAD08B07);四川省重点学科建设项目

摘　　要：研究了反相高效液相色谱法测定玉米中玉米赤霉烯酮含量的方法。玉米样品粉碎后经体积分数为90％的乙腈溶液超声提取，过滤，旋转蒸发浓缩，高效液相色谱分离，荧光检测器检测，外标法定量。对添加玉米赤霉烯酮浓度为120μg／kg的玉米样品进行了加标回收实验，回收率为87．0％-96．4％，日内相对标准偏差为5．8％，目间相对标准偏差为4．0％，该方法检出限为10μg／kg。表明该方法回收率较高，重现性较好，适合大批量检测。Determination of zearalenone in corn was studied by high performance liquid chromatography. Ground corn sample was extracted through acetonitrile solution with a volume fraction of 90% by ultrasonic wave, and then fihered, concentrated by rotary evaporation, separated by high performance liquid chromatography, detected hy a fluorescence detector and quantified by external standard method. A standard recovery test for corn sample with added zearalenone in concentration of 120 μg/kg was carried out, the recovery rate was 87.0% - 9614% , the deviation of daily inner phase to standard was 5.8% and the deviation of daily intermediate phase to standard was 4.0%. The detection limit of this method was 10μg/kg. This indicated that this method can realize a higher recovery rate and a good reproducibility, so it is suitable for detection in a large batch.

关 键 词：玉米 玉米赤霉烯酮 超声波提取 高效液相色谱法 荧光检测 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程] R115[轻工技术与工程—食品科学与工程]