RNAi干涉技术与神经生物学的实验研究  

RNA Interference Technology and Neurobiology Experimental Research

作　　者：李梅[1] 王家传[2] 吕瑞妍[1] 彭英[1] 周丽华[2] 

机构地区：[1]中山大学第二附属医院神经内科,广东广州510120 [2]中山大学中山医学院解剖学教研室,广东广州510080

出　　处：《解剖科学进展》2009年第1期111-115,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金(30672119);广东省自然科学基金(05001726;8151008901000028);广东省中医药局科技项目(1050175)

摘　　要：RNA干涉是双链RNA在细胞内介导的同源序列的mRNA转录后表达抑制,导致基因沉默。它是一种比反义技术更为有效的方法,具有更高的特异性。自从其问世以来,在肿瘤研究,遗传性疾病,基因诊断,基因治疗以及药物设计等领域得到快速发展和广泛应用。在神经生物学及神经性疾病的应用研究方面,RNA干涉技术也显示了独特的优势,如神经的发生发育过程研究及神经退行性疾病的治疗等。本文就RNA干涉技术在神经生物学领域的实验及应用研究做一综述。As a novel molecular biotechnology, RNA interference （RNAi） is emerging as a new strategy to selectively shut off the post-transcriptional expression of mRNA. The mechanism is that the homologous in sequence in cells to the silenced gene is mediated by double-stranded RNA. RNAi has more efficiency than antisense technology and has highly specificity. RNAi developed rapidly and applied extensively in fields of cancer research, genetic disorders, gene diagnosis, gene therapy and drugs designing. In aspect of neurobiology and nerve system disease application research, RNAi also has distinct dominance, such as nerve generation and development research or degenerative disease therapy .This paper has reviewed the experimental or application research of RNAi in the field of neurobiology.

关 键 词：RNA干涉技术 神经生物学 实验研究 RNAI 神经退行性疾病 MRNA转录 基因治疗 双链RNA 

分 类 号：R394[医药卫生—医学遗传学] R338[医药卫生—基础医学]