天津蓟县致病性小肠结肠炎耶尔森菌分布调查  

Investigation of Pathogenic Yersinia enterocolitica Distribution in Jixian,Tianjin

作　　者：王艳玲[1] 陈继永[1] 于凤泰 司福德 贾艳合[1] 

机构地区：[1]天津市蓟县卫生防病站疾病控制科,天津301900

出　　处：《环境与健康杂志》2009年第3期246-247,共2页Journal of Environment and Health

摘　　要：目的了解天津蓟县致病性小肠结肠炎耶尔森菌菌株分布情况。方法于2006年6月用常规方法从腹泻病患者、家畜的粪便样本中分离小肠结肠炎耶尔森菌,并进行生物化学鉴定、血清分型、相关毒力基因检测以及脉冲场凝胶电泳(PFGE)分析。结果从腹泻病患者和猪粪便中分离到的5株致病性小肠结肠炎耶尔森菌均为O:3血清型。全部携带毒力基因(ail、ystA、yadA、virF),其中4株菌经脉冲场凝胶电泳分析属于同一带型(K6GN11C30021)。另有1株带型为KGGN11C30026。结论该县从腹泻病患者与宿主动物中分离到的致病性小肠结肠炎耶尔森菌有4株属于同一个克隆系,初步推断猪为当地小肠结肠炎耶尔森菌感染人群的主要来源。Objective To get the information of the distribution of pathogenic Yersinia enterocolitica in Ji County of Tianjin. Methods Yersinia enterocolitica strains were isolated from diarrhea patients and stocks stool and the biochemical identification, serotyping, PFGE analyses were conducted, PCR was used for virulence gene detection in 2006. Results Five strains of Yersinia enterocolitica were isolated from diarrhea patients and pig stools, all of them were serotype 0：3, earring virulence genes ail, ystA, yadA,virF,and four of them had the same PFGE patterns with K6GN11C30021.Conclusion The isolates from patients and pigs belong to the same clone and pig may be the main infectious source of Yersinia enterocolitica for human beings in the county.

关 键 词：环境微生物学 小肠结肠炎耶尔森菌 基因 

分 类 号：R117[医药卫生—公共卫生与预防医学]