非霍奇金淋巴瘤组织中FGFR3和VEGF的表达及其与血管生成的相关性  

Expressions of FGFR3 and VEGF in Non-Hodgkin's Lymphoma and Their Correlations with Angiogenesis

作　　者：吴静怡[1] 曹阳[1] 孙立石[1] 刘文励[1] 周剑峰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,武汉430030

出　　处：《肿瘤防治研究》2009年第3期225-228,234,共5页Cancer Research on Prevention and Treatment

摘　　要：目的探讨非霍奇金淋巴瘤(NHL)中FGFR3和VEGF的表达与临床预后及血管生成的关系。方法用免疫组化Envision法检测62例NHL和40例反应性增生淋巴结(RLH)组织中FGFR3、VEGF和CD105的表达情况。结果FGFR3在36例NHL组织中有表达,阳性率为58.06%(36/62)。而40例RLH组织仅有5%(2/40)表达FGFR3。CD105的表达在NHL和RLH组织中差异有统计学意义(P<0.05)。VEGF在NHL组织中高表达(75.81%),并与NHL的惰性或侵袭性分组和国际预后指数(International Prognostic Factors,IPI)呈显著相关(P<0.05)。同时FGFR3和CD105表达均与NHL的临床分期、结外侵犯和IPI呈显著性相关(P<0.05和P<0.001)。等级相关分析发现在NHL组织中FGFR3与VEGF和MVD均呈显著正相关(r=0.326,P<0.05和r=0.567,P<0.001)。结论NHL组织中FGFR3过表达及其与VEGF和MVD的正相关性提示FGFR3很可能通过上调VEGF促血管生成从而参与肿瘤形成。Objective To investigates the relationship importance of FGFR3 and VEGF, in prognos and angiogenesis in patients with Non-Hodgkin＇s lymphoma（NHL）. Methods Expression of FGFR3, VEGF and CD105 in 62 specimens of NHL and 40 specimens of reactive lymphoid hyperplasia （RLH） were detected by immunohistochemistry Envision. Results Positive rate of FGFR3 expression was 58. 06% in NHL tissue, and 5% in RLH tissue. The expression of CD105 was significant difference between NHL and RLH tissue（P〈0. 05）. VEGF was over-expression in NHL tissue （75.81% ） and dramaticly related with histology grade （indolent or aggressive） and International Prognostic Factors （IPI） （P〈0. 05）. FGFR3 and CD105 staining in NHL tissue were significant correlation with clinical stage, extronodal infiltration and IPI （P〈0. 05 and P〈0. 001）. The Spearman rank correlation assay indicated, positive correlation of FGFR3 with both VEGF （r= 0. 326,P%0. 05） and CD105 （r= 0. 567,P%0. 001） expression in NHL. Conclusion Over expression of FGFR3 and its positive correlation with VEGF and CD105 in NHL tissue revealed that FGFR3 maybe up-regulate the expression of VEGF which involved in angiogenesis and invasion of NHL.

关 键 词：非霍奇金淋巴瘤 FGFR3 免疫组织化学 微血管密度 CD105 VEGF 

分 类 号：R733.4[医药卫生—肿瘤]