电压门控钠通道在卵巢癌中功能性表达的研究  

Voltage-gated sodium channels Nav1.5 functionally expressed in ovarian caner

作　　者：高瑞[1] 沈怡[1] 徐舒翔[1] 雷鸣[2] 王泽华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉430022 [2]华中科技大学同济医学院附属协和医院心内科离子通道病研究中心,武汉430022

出　　处：《现代妇产科进展》2009年第2期117-120,共4页Progress in Obstetrics and Gynecology

基　　金：国家博士后基金(No:20080440946)

摘　　要：目的:探讨电压门控钠通道(voltage-gated sodium channels,VGSC)不同亚型表达及与卵巢癌转移的关系。方法:以人卵巢癌细胞和卵巢肿瘤组织标本为研究对象,用RT-PCR,激光共聚焦,MTT及Transwell小室法,检测卵巢癌细胞中VGSC表达的亚型及VGSC特异性阻断剂河豚毒素(tetrodotoxin,TTX)对卵巢癌体外侵袭性的影响。结果:卵巢癌高转移细胞系(CAOV3,A2780,SKOV3)中高表达的VGSC亚型Nav1.5与卵巢癌的恶性生物学特性明显相关;30μmol/L TTX对CAOV3,A2780,SKOV3细胞体外的侵袭力的抑制率约为50%,差异有统计学意义(P<0.05),对不表达Nav1.5的低转移卵巢癌细胞Anglne的转移级联过程则没有明显影响。结论:VGSC亚型Nav1.5与卵巢癌的体外转移明显相关,可能成为卵巢肿瘤的潜在标记物和治疗靶点。Objectlve：To explore the effect of Voltage-gated sodium channels（VGSCs） subtypes on metastasis behavior of human ovarian cancer cell lines and ovarian cancer tumor tissues. Methods：RT-PCR, SBFI confacol, MTT and Transwell assays were respectively used to detect the functional expression of VGSC subtypes in ovarian cancer cells and the effect of specific Voltage-gated sodium channels inhibitor tetrodotoxin （TTX） on invasion of ovarian cancer in vitrol Results：The mRNA expression of Nav1. 5 in ovarian cancer cell lines was correlated with oncogenic properties of ovarian cancer. 30umoL/L TTX could sharply decrease the 50% invasion of strongly metastatic ovarian cancer cells （ CAOV3, A2780, SKOV3 ） by inhibiting the inflow in these cell lines （ P 〈 0.05 ）. TTX had no obvious changes cell Anglne which was nearly no Navl. 5 expression. Conclusion：The up on weakly metastatic -regulation of VGSC NaV1.5 in ovarian cancer is correlated with ovarian cancer metastasis in vitro. Nav1. 5 may be a potential novel marker for human ovarian cancer.

关 键 词：电压门控钠通道 NAV1.5 肿瘤侵润 卵巢肿瘤 河豚毒素 

分 类 号：R737.31[医药卫生—肿瘤]