甜瓜EST序列中微卫星的分布特征  

Characteristics of Microsatellites in Melon(Cucumis melo L.) EST Sequences

作　　者：胡建斌[1] 刘颖[1] 王兰菊[1] 李建吾[1] 

机构地区：[1]河南农业大学园艺学院,郑州450002

出　　处：《植物生理学通讯》2009年第3期258-262,共5页Plant Physiology Communications

基　　金：国家"863"计划(2007AA10Z100);河南农业大学博士启动基金(30400247)

摘　　要：GenBank中35547条甜瓜EST经去冗余处理后,得到总长度为250.3Mb的无冗余EST34438条。这些序列中有2813个微卫星简单重复序列(Simple sequence repeat,SSR),分布于2107条EST中,出现频率为8.16%,平均分布距离为8.90kb。三核苷酸重复是主导重复类型,占SSR总数的47.14%;其次是二核苷酸和单核苷酸重复,分别占SSR总数的20.72%和16.99%。AAG/TTC是优势重复基元,占微卫星总数的29.26%,AG/CT和A/T分别占14.61%和16.25%。在所有的SSR中,重复次数为4～10次的占70.32%,长度为12～20bp的占51.12%。并对这些SSR的多态性潜能进行了评价。34 438 melon expressed sequence tags （ESTs） were obtained after the removal of redundant sequences from 35 547 melon ESTs deposited in GenBank. In these ESTs, 2 813 microsatellites （simple sequence repeat, SSR） were tested and distributed in 2 107 ESTs. The occurrence frequency of SSR was 8.16% and the average distribution distance was 8.90 kb. Trinucleotide repeats were dominant repeat types and accounted for 47.14%. Dinucleotide and mononucleotide repeats accounted for 20.72% and 16.99%, respectively. AAG/TTC was most frequent repeat motif and accounted for 29.26% in all SSRs. AG/CT and A/T accounted for 14.61% and 16.25%, respectively. Among all SSRs, SSRs which repeated 4 to 10 times accounted for 70.32%, and SSRs which was 12 to 20 bp long accounted for 51.12%. The potential polymorphism of these S SRs was also analyzed.

关 键 词：甜瓜 EST序列 微卫星 特征 

分 类 号：S652[农业科学—果树学]