混合菌种利用淀粉在光照及黑暗条件下的产氢  

HYDROGEN PRODUCTION FROM STARCH USING MIXED CULTURES UNDER DARK AND LIGHT CONDITION

作　　者：张立宏[1] 程军[1] 陈明[1] 周俊虎[1] 刘建忠[1] 岑可法[1] 

机构地区：[1]能源清洁利用国家重点实验室(浙江大学),杭州310027

出　　处：《太阳能学报》2009年第3期275-281,共7页Acta Energiae Solaris Sinica

基　　金：国家自然科学基金(50406022);国家高技术研究发展计划(863)项目(2006AA05Z122);全国优秀博士学位论文作者专项资金(200437);霍英东高等院校青年教师优选资助项目(104021);浙江省科技攻关计划项目(2006C33049)

摘　　要：进行了混合菌种利用淀粉进行光反应和暗反应的产氢对比试验。结果发现光合反应的产氢率以及产氢速率均高于暗反应,但产氢时间滞后于暗反应。对反应的菌液进行了DNA提取、纯化以及PCR扩增,通过对DGGE图谱分析,发现光反应和暗反应的样品条带在数量和亮度上都存在一定差异,暗反应条件下产氢的优势菌群要略多于光合反应的菌群。随着淀粉浓度的增加,光合反应和暗反应的产氢总量均增大,但产氢率降低,其中光合反应的降低尤为明显,从2g/L淀粉浓度时的9.8mmol H_2/g淀粉降低到40g/L淀粉浓度时的3.3mmol H_2/g淀粉,产氢率降低了66%,而暗反应产氢率降低了43%。光合反应的尾液中,丁酸含量最高,而暗反应的尾液中,乙酸含量最高。Hydrogen production from starch by mixed cultures under light and dark conditions was studied in batch experiments. Results showed that hydrogen yield and hydrogen production rate under light condition higher than those under dark condition, but the lag time under light condition was longer than that under dark condition. Analysis of DGGE profile showed that the quantity and brightness of bands were different under light and dark condition, predominant microbial population in quantity under dark condition was more. With starch concentration increasing, the cumulative hydrogen production increased and hydrogen yield decreased. The decrease under light condition was more distinct, from 9.8mmol H2/g starch at starch concentration of 2g/L decreased to 3.3mmol H2/g starch at starch concentration of 40g/L. Butyrate and acetate was the main acid product of the effluent under light and dark condition, respectively.

关 键 词：产氢 混合菌种 淀粉 光反应 暗反应 

分 类 号：TN511.4[电子电信]