聚邻苯二胺固定葡萄糖氧化酶在微带金电极上的性质  

Characteristics and Application of Glucose Oxidase Immobilized by Poly(OPhenylenediamide) Film at NanometerSized Microband Gold Electrode

作　　者：汪云[1] 周东美[2] 陈洪渊[2] 

机构地区：[1]无锡轻工大学化学工程系 [2]南京大学化学化工学院

出　　处：《无锡轻工大学学报（食品与生物技术）》1998年第1期69-73,共5页Journal of Wuxi University of Light Industry

摘　　要：用电化学方法在纳米级微带金电极上聚合邻苯二胺（ＰＰＤ）的同时实现了葡萄糖氧化酶（ＧＯＤ）的固定。制得的ＧＯＤ－ＰＰＤ－Ａｕ酶电极呈现典型的酶催化反应动力学特征，具有抗干扰、响应速度快、稳定性好的优点，固定化葡萄糖氧化酶的表观米氏常数为５．４ｍｍｏｌ／Ｌ．Glucose oxidase was simultaneously immobilized in polymer film at a nanometersized microband gold electrode by means of electrochemical polymerization of ophenylenediamide. The response to glucose shows kinetic characteristics of the typical enzymecatalyzed reaction at GODPPDAu. Moreover, it possesses many virtures such as resistance, rapid electrochemical response and good stability. The apparent Michealis Constant is 5.4mmol/L.

关 键 词：电化学 微带金电极 聚邻苯二胺 葡萄糖氧化酶 

分 类 号：TQ925.4[轻工技术与工程—发酵工程] O657.1[理学—分析化学]