原发性肝癌中N-ras、c-myc癌基因的表达研究  

A Study on Oncogenic Expressions of N-ras and c-myc in Primary Carcinoma of the Liver

作　　者：冷静[1] 彭韬[1] 徐秉栋[1] 印虹[1] 陆培新 王能进 张松平 张瑞菊 

机构地区：[1]南京医科大学病理教研室,210029 [2]启东市肝癌研究所

出　　处：《江苏医药》1998年第6期387-389,共3页Jiangsu Medical Journal

基　　金：江苏省自然科学基金

摘　　要：以点杂交和原位杂交法检测了34例启东地区原发性肝癌手术切除标本N－ras、c－myc基因的表达水平和定位。点杂交结果显示：N－ras、c-myc的检出率分别为647％（22／34）和23．5％（8／34）。前期的实验表明此34例标本HBV－DNA的检出率为588％（20／34），Southernblot实验结果未见病毒DNA的整合。N－ras、c-myc阳性标本中HBV－DNA的检出率分别为54．5％（12／22）和75％（6／8），两者之间无差异，P＞0．05。原位杂交结果显示：肝癌癌组织中N－ras、c－myc基因的表达水平并没有明显增高，相反，肿瘤周围肝组织中的表达水平增高并主要位于慢性肝炎、肝硬变中的再生肝细胞中，阳性细胞呈灶性及成片分布，不伴有毛玻璃样改变（HE染色）。结论：虽然N－ras、c－myc与肝癌的发生密切相关，肝癌患者中常见其表达水平增高，但这很可能只是一种肝细胞损伤后再生时的细胞增殖性调控的表现，此种N－ras、c-myc的高表达是否直接导致肝癌发生，癌基因是否为突变型，有待进一步研究、此外，本实验中c－myc基因相对于N－ras基因的低检出率可能与c－mycMRNA极短的半衰期有关。34 cases of primary liver carcinoma from Qitung city, a high liver cancer risk region in China, hadbeen examined by dot and in situ hybridization tests for studying the expression and location of N-ras and c-myconcogenes. Dot hybridization test showed that the positive expressions of N-ras and c-myc were 64.7% (22/34)and 23. 5% (8/34) respectively. Besides, HBV-DNA was found in 58. 8% (20/34) of these cases and 12 out of 22(54.5 % ) in N-ras positive cases, 6 out of 8(75%) in c-myc positive cases, the difference was insignificant (P>0. 05). No HBV-DNA was found integrated into hepatic genome by Southern blot hybridization by out previousstudy. In situ hybridization tests showed indistinct expressions of N-ras and c-myc in cancerous region and over-expressive level in cancer-surrounding tissues especia1ly the proliferous hepatocytes of chronic hePatitis and cirrho-sis. The positive cells were in spotty and focal distribution without 'ground glass' changes (by HE stain). Wesupposed that although there were great relations between over-expression of N-ras,c-myc oncogenes and primaryliver carcinoma, it is possible that it was.simply a phenomenon associated with the proliferative gene control whenpost-injury regeneration initiated- It is still unknown if these over-expressive genes were mutated- In addition, thelower positive rate of c-myc expression as compared to that of N-ras in our experiment was probably due to the ex-tremely short half-life time of c-myc mRNA.

关 键 词：肝肿瘤 原发性 N-RAS C-MYC 原位杂交 癌基因 

分 类 号：R735.7[医药卫生—肿瘤]