采用荧光底物法检测纯生啤酒蛋白酶A方法的研究  

Study on the Detection of Protease A Activity in Draft Beer by Fluorescent Substrate

作　　者：陈旭[1,2] 王德良[1,2] 杨海燕[1] 王晓娟[1] 王志萍[1] 

机构地区：[1]新疆农业大学食品科学学院,新疆乌鲁木齐830052 [2]中国食品发酵工业研究院酿酒工程部,北京100027

出　　处：《酿酒科技》2009年第4期108-110,113,共4页Liquor-Making Science & Technology

基　　金："十一五"国家科技支撑计划项目(2007BAK36B00)"我国优势传统食品啤酒制造业关键技术研究与应用"子课题--纯生啤酒泡沫稳定性研究与应用

摘　　要：对蛋白酶A的荧光检测方法进行了研究。分析了标准蛋白酶A稀释液对标准曲线建立的影响、检测方法的稳定性及检测时间对样品结果的影响。并对蛋白酶A酶活力与荧光值的相关性进行了分析,证明了在同一批样品检测中蛋白酶A酶活与对应的荧光值具有很好的相关性,可以根据荧光值的大小直接判断和比较蛋白酶A活力的高低。In this paper, the fluorescent detection method of protease A was studied. The effects of standard protease A dilution on the establishment of standard curve were analyzed, and the effects of the detection method stability and detection time on sample testing results were investi- gated. Besides, the relevance ofprotease A activity and fluorescence values was analyzed. The results showed that protease A activity in the same batch of samples had good correlations with the corresponding values of the fluorescence. Accordingly, protease A activity could be judged and compared based on the values of fluorescence.

关 键 词：纯生啤酒 蛋白酶A 荧光底物 

分 类 号：TS262.5[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]