羧甲基-β-环糊精用于若干手性药物的毛细管电泳拆分  

Enantiomeric Separation of Basic Drugs with Carboxymethylβcyclodextrin by Capillary Electrophoresis

作　　者：李学仁[1,2] 程庆春[1,2] 杨跃伟 张云斌 尚素芬 王洪[1,2] 

机构地区：[1]解放军第二五二医院 [2]河北大学化学系

出　　处：《中国药科大学学报》1998年第3期210-213,共4页Journal of China Pharmaceutical University

摘　　要：以羧甲基β环糊精（ＣＭβＣＤ）为手性选择剂，利用毛细管电泳（ＣＥ）法对五种手性药物进行了成功的拆分，并与β环糊精（βＣＤ）进行了对比。对影响手性分离的主要因素：手性选择剂、背景电解质（ＢＧＥ）、分离体系的酸度和温度进行了讨论，并对手性识别机理进行了探讨。Capillary electrophoresis was used for the chiral separation of racemic chlorprenaline, salbutamolum, terbutaline, orciprenaline and clenbuterolum with βcyclodextrin (βCD) and 2,6diOcarboxymethylβcyclodextrin (CMβCD) as chiral selectors employing an uncoated capillary. The βCD types and concentrations have strong effect on the chiral separation. The effects of the pH and composition of the background electrolyte (BGE), the amphiphilic compound in the BGE and the capillary temperature on the mobility and chiral separation have been studied. CMβCD gave a baseline enantiomeric separation for the basic drugs under study, whereas the βCD, under the same experimental conditions, was poor in stereoselectivity and gave just partial chiral separation. The tetraethylammonium bromide and tetrabutylammoniun bromide added to the BGE could improve the chiral separation but the hexadecyltrimethylammonium bromide made the chiral separation worse while using βCD as chiral selector. Lower capillary temperatures were more favorable for the chiral resolution of βCD and no significant effect was found for CMβCD.

关 键 词：羧甲基 Β-环糊精 手性药物 毛细管电泳 

分 类 号：R914.1[医药卫生—药物化学]