应用非同位素 PCR-SSCP 检测膀胱癌组织中 p53 基因变异的初步研究  

PRELIMINARY STUDY ON MUTATION OF p53 GENE IN BLADDER CANCER BY NONISOTOPIC PCR SSCP

作　　者：赵温利[1,2] 陈钦忠[1,2] 崔树龄[1,2] 温培娥 崔正言[1,2] 

机构地区：[1]山东省医学科学院基础所免疫室 [2]山东医科大学附属医院泌尿外科

出　　处：《山东医科大学学报》1998年第1期5-7,共3页Acta Academiae Medicinae Shandong

基　　金：国家自然科学基金

摘　　要：应用聚合酶链式反应—单链构像多态性分析（ＰＣＲ－ＳＳＣＰ）银染色法检测了３９例膀胱移行上皮癌组织中ｐ５３基因的突变率为３０．８％，其中５、６、７、８外显子的突变率分别为７．７％、５．１％、５．１％、１２．８％；病理分级的Ⅰ、Ⅱ、Ⅲ级肿瘤组织中ｐ５３基因突变率分别为７．１％、３０．０％、５３．３％（Ｐ＜０．０５）。提示ｐ５３基因突变在膀胱肿瘤的衍变过程中可能起重要作用，可能是致其恶性程度提高的继发事件；ＰＣＲ－ＳＳＣＰ银染法是一种快速、简单且安全的检测基因突变的方法，具有推广应用价值。p53 gene mutations in exons 5-8 were detected in 39 cases of bladder cancer by nonisotopic PCR SSCP.The results showed that 12 cases had mutation(30.8%),of which 3 in exon 5(7.7%),2 in exon 6(5.1%),2 in exon 7(5.1%)and 5 in exon 8( 12.8 %).With reference to clinical pathological features,the positive rates of p53 gene mutations in grade Ⅰ、Ⅱ and Ⅲ were 7.1%、30.0% and 53.3% respectively( P <0.05).The results suggest that the mutations of p53 may play a role in the pathogenesis of bladder cancer and that nonisotopec PCR SSCP technique is especially useful in the screening of gene mutation in clinical practice.

关 键 词：膀胱肿瘤 P53基因 PCR 基因变异 

分 类 号：R737.140.2[医药卫生—肿瘤]