木醋杆菌ITS序列分析及产纤维素条件的研究  

ANALYSIS OF ITS SEQUENCE IN ACETOBACTER XYLINUM AND RESEARCH THE CONDITION OF PRODUCTION FIBRE

作　　者：张艳玲[1] 石晓珍[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《食品研究与开发》2009年第4期1-6,共6页Food Research and Development

基　　金：国家科技支撑计划项目(2007BAD48B02);中央级公益性科研院所基本科研业务费专项(2007hzs1J007);科技部"国家技术基础条件平台工作"(2005DKA21201)

摘　　要：木醋杆菌(Acetobacter xylinum)是合成纤维素能力最强的细菌,设计木醋杆菌16S-23SrDNA转录间隔区(internally transcribed spacer ITS)序列引物,对其扩增。在GenBank上的登陆号为GenBank EU196159。选择到合适的木醋杆菌ITS扩增引物,PCR扩增条件。木醋杆菌最佳培养温度28℃,通过测定纤维素产量发现最佳接种量5%,最佳碳源为葡萄糖,最佳碳源浓度为2.5%。Acetobacter xylinum is a bacteria which has the most capable of synthetic fibre,this experiment designed the primers of internally transcribed spacer ITS in Acetobacter xylinum, and the generic PCR products from the ITS were cloned and sequenced. The result is compared in NCBI BLAST and analysed in DNA STAR, the landing number in GenBank is EU196159. Simultaneity have choice the appropriate primer of ITS, PCR condition. According to cellulose production we found that the optimization cultivate temperature is 28 ℃, inoculability quantity is 5 %, optimization carbon source is glucose, optimization carbon source density is 2.5 %.

关 键 词：木醋杆菌 ITS 纤维素 产量 

分 类 号：TQ920[轻工技术与工程—发酵工程]