水源水中2-MIB降解菌的筛选  

Isolation of a bacterium capable of removing 2-methylisoborneol from water

作　　者：周北海[1] 王俊[1] 蔡珉敏[1] 祝玲 宋文娟 

机构地区：[1]北京科技大学土木与环境工程学院,北京100083

出　　处：《北京科技大学学报》2007年第S2期227-230,共4页Journal of University of Science and Technology Beijing

基　　金：北京市科委资助项目(NoD0605004040421);北京科技大学本科生创新基金(SRTP)资助项目

摘　　要：针对特定水处理工艺,研究了投加2-MIB前后水中微生物种群的变化,建立了筛选分离2-MIB降解菌的方法.以生物膜生长成熟的活性炭连续处理含有较高浓度2-MIB水样,然后从活性炭表面取样,用2-MIB为惟一碳源的无机盐培养基筛选和驯养,最后进行划线分离,成功得到2-MIB降解菌种.通过16SrRNA分析,该菌株属于假单胞菌属(Pseudomonas spp.).For the given water treatment,the features of the microbiological colonies before and after the addition of the 2-methylisoborneol(2-MIB) were studied,and a method for isolation of a bacterium capable of removing the 2-MIB from the water was proposed.The procedure of the method was as follows:the activated carbon,whose surface was covered by a mature microbiological membrane,was employed to continually treat the water with higher concentration of 2-MIB,and then the sample gained from the surface of the activated carbon was cultivated in the mineral salt liquid culture in which 2-MIB was the only carbon source;finally,one strain,which was identified as Pseudomonas spp.based on the data of 16S rRNA analysis,was isolated by plate streaks.

关 键 词：水源水 2-MIB 菌种筛选 假单胞菌属 

分 类 号：TU991.2[建筑科学—市政工程]