Research of the relationship of intracellular acidification and apoptosis in Hela cells based on pH nanosensors  

作　　者：HE XiaoXiao WANG Yan WANG KeMin PENG JiaoFeng LIU Fang TAN WeiHong 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry&Chemical Engineering,Institute of Bio-logical Technology,Hunan University,Changsha 410082,China [2]Engineering Center for Biomedicine,Hunan University,Changsha 410082,China [3]Institute of Life Science and Biological Technology,Hunan University,Changsha 410082,China [4]Engineering Research Center for Bio-Nanotechnology of Hunan Province,Changsha 410082,China

出　　处：《Science China Chemistry》2007年第2期258-265,共8页中国科学（化学英文版）

基　　金：Supported by the Key Project of National Natural Science Foundation of China (Grant No. 20135010);the National Key Basic Research Program of China (Grant No. 2002CB513100-10);the Key Technology Research and Development Program of China (Grant No. 2003BA310A16);the High-Tech Research and Development Program of China (Grant No. 2003AA302250);the International Cooperation Key Project of Science and Technology Ministry (Grant No. 2003DF000039);the National Natural Science Foundation of China (Grant No. 20405005)

摘　　要：In this paper,the relationship of intracellular acidification and apoptosis in Hela cells induced by vin-cristine sulfate has been studied by use of the ratiometric pH nanosensors that have been developed by our group,employing fluorescein isothiocyanate(FITC) doped as the pH-sensitive dye and Tris(2,2'-bipyidyl) dichlororuthenium(II) hexahydrate(RuBpy) doped as reference dye. The pH change of the Hela cells induced by vincristine sulfate has been monitored in vivo,in situ and real time by use of the ratiometric pH nanosensors. The experimental results show that the pH of the apoptotic Hela cells induced by vincristine sulfate has been acidified from 7.11 to 6.51,and the percentage of intra-cellular acidification is correlated with the induced concentration and incubation time of the vincristine sulfate. The further study of the percentage of intracellular acidification and the percentage of apop-tosis of Hela cells at the same time reveals that apoptosis of Hela cells induced by vincristine sulfate is preceded by intracellular acidification. These results would provide theoretical foundation for the therapy of cancer through interfering the pH of cells by use of vincristine sulfate or other anti-cancer drugs.In this paper, the relationship of intracellular acidification and apoptosis in Hela cells induced by vincristine sulfate has been studied by use of the ratiometric pH nanosensors that have been developed by our group, employing fluorescein isothiocyanate (FITC) doped as the pH-sensitive dye and Tris(2,2′-bipyidyl) dichlororuthenium(II) hexahydrate (RuBpy) doped as reference dye. The pH change of the Hela cells induced by vincristine sulfate has been monitored in vivo, in situ and real time by use of the ratiometric pH nanosensors. The experimental results show that the pH of the apoptotic Hela cells induced by vincristine sulfate has been acidified from 7.11 to 6.51, and the percentage of intracellular acidification is correlated with the induced concentration and incubation time of the vincristine sulfate. The further study of the percentage of intracellular acidification and the percentage of apoptosis of Hela cells at the same time reveals that apoptosis of Hela cells induced by vincristine sulfate is preceded by intracellular acidification. These results would provide theoretical foundation for the therapy of cancer through interfering the pH of cells by use of vincristine sulfate or other anti-cancer drugs.

关 键 词：RATIOMETRIC PH nanosensors VINCRISTINE sulfate Hela CELLS intracellular ACIDIFICATION APOPTOSIS of CELLS 

分 类 号：Q255[生物学—细胞生物学]