Protamex复合蛋白酶水解大豆分离蛋白的研究  

Study on hydrolysis of SPI by protamex protease

作　　者：刘艳秋[1] 陈光[1] 

机构地区：[1]吉林农业大学生物技术学院,吉林长春130118

出　　处：《吉林工程技术师范学院学报》2005年第3期9-13,共5页Journal of Jilin Engineering Normal University

基　　金：吉林省科学技术厅资助(20020211)

摘　　要：为进一步优化大豆分离蛋白酶水解条件,本试验采用二次回归正交旋转组合设计方法对Protamex复合蛋白酶水解大豆分离蛋白的条件进行研究。建立了水解度(DH)与pH值、反应温度、反应时间、底物浓度、酶与底物浓度比之间的数学模型;并获得最佳水解工艺条件:pH值6.5,温度40℃,水解时间10hr,底物浓度14%,酶与底物浓度比4.5%;主成分分析表明,pH对DH的贡献率最大。In this thesis, for optimizing the hydrolysis condition of soybean protein isolated (SPI) by Protamex protease, second-order regression rotation designing has been used. After establishing the mathematics matrix between the degree of[JP2] hydrolysis (DH) and five factor(pH value, hydrolysis time, substrate concentration, hydrolysis temperature , the ratio of enzyme and substrate),and determing the high yield scheme and the optimization design,the author gets optimization condition: hydrolysis time 10hr., substrate concentration at 14%,the ratio of enzy me and substrate at 4.5%, pH6.5 and 40℃,and concludes that pH value[JP] contributes to DH mostly.

关 键 词：回归旋转设计 大豆分离蛋白 PROTAMEX复合蛋白酶 水解 

分 类 号：TQ936[化学工程]