Inhibitory effect of nomegestrol acetate on steroidogenesis of cultured granulosa celis from rat ovary in vitro  

诺美孕酮醋酸盐对离体培养大鼠卵巢颗粒细胞分泌甾体激素的抑制作用(英文)

作　　者：钱立晖[1] 杨波[1] 冷颖[1] 曹霖 顾芝萍[1] 

机构地区：[1]中国科学院上海生命科学研究院上海药物研究所,上海中国200031

出　　处：《Acta Pharmacologica Sinica》2001年第1期42-46,共5页中国药理学报（英文版）

摘　　要：AIM: To study the effect of nomegestrol acetate, a new synthetic progesterone on granulosa cells' viability and steroidogenesis function. METHODS: Granulosa cells were cultured in McCoy's 5A medium. Trypan blue stain was used to measure viable cells. FSH and testosterone were added to stimulate the steroid secretion. Specific RIA assay was used to evaluate the estrogen and progesterone secretion respectively. RESULTS: IC50 of nomegestrol acetate to damage cells is 6.85 mg/L (95 % confidence limits 5.36 - 8.75 mg/L). Nomegestrol acetate 0.45, 0.9, and 1.8 mg/L greatly inhibited the estrogen secretion from granulosa cells by 7.6 %, 12.5 %, 28.3 % in the presence of testosterone 0.5 μmol/L and FSH 10 U/L without affecting the number of viable cells. The secretion of progesteron were markedly decreased by 44.5 % , 53.3 % , and 62.0 % concurrently. CONCLUSION: Nomegestrol acetate directly inhibited the steroidogenesis of granulosa cells.目的:观察诺美孕酮对离体培养大鼠颗粒细胞分泌雌、孕激素功能的抑制作用。方法:台盼蓝排斥法进行活细胞计数。加入FSH和睾酮刺激颗粒细胞激素分泌。放免法测定培养液中雌、孕激素含量。结果:诺美孕酮杀伤细胞的IC_(50)为6.85mg/L(95％可信限:5.36-8.75mg/L)。诺美孕酮0.45,0.9,和1.8mg/L在不影响活细胞数的情况下对颗粒细胞分泌雌激素的抑制率分别为7.6％,12.5％和28.3％,对其分泌孕激素的抑制率分别为44.5％,53.3％和62.0％。结论:诺美孕酮直接抑制离体培养的大鼠颗粒细胞分泌雌、孕激素。

关 键 词：ESTROGEN PROGESTERONE cultured cells nomegestrol OVARY 

分 类 号：R96[医药卫生—药理学]