Genomic Organization and Expression in E. coli of Zebrafish Terra  

作　　者：赵知行 华正春 孟安明 

出　　处：《Tsinghua Science and Technology》2001年第3期265-268,共4页清华大学学报（自然科学版（英文版）

基　　金：the National Natural Science Foundationof China (No.39970 36 0 and No.30 0 2 5 0 2 0 ) ;theNational Key Basic Research and Developm entProgram (No.G19990 75 6 )

摘　　要：Zebrafish terra encodes a transcription factor that is specifically expressed in developing somites. Previous studies suggested that this gene is involved in vertebrate somitogenesis. In this study, the genomic DNA of terra locus was isolated and its organization was investigated. The analysis showed that terra locus consists of 3 introns and occupies 3154 bp in the genome of zebrafish. The exon intron junctions of the second and third introns conform to the GT AG rule, while the first intron has the unusual junction sequences of GT AC. An IPTG inducible expression system was established to produce terra protein in bacterial cells. Overexpression of terra protein leads to the formation of inclusion bodies in the bacterial cells. The protein will be used to study its structure and function. Zebrafish terra encodes a transcription factor that is specifically expressed in developing somites. Previous studies suggested that this gene is involved in vertebrate somitogenesis. In this study, the genomic DNA of terra locus was isolated and its organization was investigated. The analysis showed that terra locus consists of 3 introns and occupies 3154 bp in the genome of zebrafish. The exon intron junctions of the second and third introns conform to the GT AG rule, while the first intron has the unusual junction sequences of GT AC. An IPTG inducible expression system was established to produce terra protein in bacterial cells. Overexpression of terra protein leads to the formation of inclusion bodies in the bacterial cells. The protein will be used to study its structure and function.

关 键 词：ZEBRAFISH TERRA INTRON EXON EXPRESSION bacterial cells 

分 类 号：Q786[生物学—分子生物学]