Analysis of the chemotactic activities of mouse chemokine MIP-2 to thymocyte subgroups  

作　　者：Gong Shunyou Gao Ling Qian Xiaoping Tian Tian Chen Weifeng 

机构地区：[1]Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

出　　处：《Chinese Science Bulletin》2001年第20期1694-1698,共5页

基　　金：This work was supported by the International Center for Genetic Engineering and Biotechnology (ICGEB) (Grant No. 98/016) ;and the National "973" Project of China (Grant No. G1999053904).

摘　　要：A mouse chemokine MIP-2(macrophage in-flamatory protein 2) is constitutively expressed not only by peritoneal macrophages, but also by fresh thymic stromal cells, based on RT-PCR detection. Moreover, the specific receptor of MIP-2 is expressed at different levels among four main subgroups of murine thymocytes including DN, DP, CD4SP and CD8SP. By the chemotaxis assays with Boyden chamber, we proved that the recombinant mouse MIP-2 can chemoattract the four main subgroups of thymocytes in different degrees, it mainly chemoattract the DP and SP subgroups. We firstly reported that MIP-2 is involved in the regulation of the directional migration of developing thymocytes.A mouse chemokine MIP-2 (macrophage inflamatory protein 2) is constitutively expressed not only by peritoneal macrophages, but also by fresh thymic stromal cells, based on RT-PCR detection. Moreover, the specific receptor of MIP-2 is expressed at different levels among four main subgroups of murine thymocytes including DN, DP, CD4SP and CD8SP. By the chemotaxis assays with Boyden chamber, we proved that the recombinant mouse MIP-2 can chemoattract the four main subgroups of thymocytes in different degrees, it mainly chemoattract the DP and SP sub-groups. We firstly reported that MIP-2 is involved in the regulation of the directional migration of developing thymocytes.

关 键 词：MACROPHAGE inflamatory protein 2 THYMOCYTES chenio-tactic activity. 

分 类 号：Q952[生物学—动物学]